Technical note: double immunohistochemical stain (anti-CD31 and anti-cytokeratins) as a tool for a confident forensic post-mortem diagnosis of amniotic fluid embolism
| Autor: | Antonio Tombolini, Gerardo Ferrara, Irene Broglia |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Embolism Amniotic Fluid CD31 Pathology medicine.medical_specialty Amniotic fluid Stain Pathology and Forensic Medicine Amniotic fluid embolism Pregnancy medicine Humans Forensic Pathology Lung Fetus Staining and Labeling business.industry Microangiopathy Endothelial Cells medicine.disease Immunohistochemistry Platelet Endothelial Cell Adhesion Molecule-1 medicine.anatomical_structure Keratins Female business |
| Zdroj: | International Journal of Legal Medicine. 135:355-357 |
| ISSN: | 1437-1596 0937-9827 |
| DOI: | 10.1007/s00414-020-02331-y |
| Popis: | Amniotic fluid embolism (AFE) is a rare cause of unexpected late maternal gestational death. The forensic post-mortem diagnosis is rendered upon the histological recognition of fetal "foreign" material inside maternal lung vasculature. The authors propose a double immunohistochemical (anti-CD31 plus anti-cytokeratin AE1/AE3) stain in order to assess accurate amniotic fluid pulmonary embolic burden in a highly reproducible fashion based on the fact that such technique allows to detect an impressive amount of scales within lung vasculature, thereby offering further evidence that pulmonary embolic obstructive microangiopathy, rather than anaphylactoid reaction, is major determinant in AFE-related death. |
| Databáze: | OpenAIRE |
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