An enzyme-linked immunosorbent assay-based isolation procedure for verotoxigenic Escherichia coli
| Autor: | L H Sekla, D G Milley |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Serotype
Meat food.ingredient medicine.drug_class Polymyxin Bacterial Toxins Enzyme-Linked Immunosorbent Assay Shiga Toxin 1 medicine.disease_cause Monoclonal antibody Applied Microbiology and Biotechnology Microbiology Feces fluids and secretions food Escherichia coli medicine Animals Humans Agar Ecology biology Toxin biology.organism_classification Enterobacteriaceae Molecular biology Cattle Bacteria Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 59:4223-4229 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.59.12.4223-4229.1993 |
| Popis: | A colony enzyme-linked immunosorbent assay using the hydrophobic grid membrane filter format was developed for the isolation of verotoxigenic Escherichia coli from human and food samples. The method utilizes monoclonal antibodies directed against the verotoxins and is sensitive to all verotoxin 1- and/or 2-producing serotypes. E. coli that produced a minimum of 2 x 10(2) and 2 x 10(3) 50% cytotoxic doses per ml of verotoxins 1 and 2, respectively, were detectable. In a method comparison using human stool specimens, this procedure isolated 29% more E. coli O157 than did the standard sorbitol-MacConkey agar procedure, with no false-positive reactions. When applied to meat, 11 of 20 samples positive for verotoxin by polymyxin extraction yielded verotoxigenic E. coli of a variety of serotypes including O157:H7. Four false positives were noted. This procedure provides a sensitive means for the isolation of verotoxigenic E. coli and should facilitate recovery of those serotypes that are otherwise indistinguishable from nonpathogenic strains. |
| Databáze: | OpenAIRE |
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