An Automated Microfluidic System for Morphological Measurement and Size-Based Sorting of C. Elegans
| Autor: | Pengfei Song, Xianke Dong, Xinyu Liu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Software_OPERATINGSYSTEMS
Microfluidics Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Size measurement Body size Culture Techniques Image Processing Computer-Assisted Animals Electrical and Electronic Engineering Caenorhabditis elegans business.industry Sorting Equipment Design Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Sizing Computer Science Applications Nematode worm ComputingMilieux_MANAGEMENTOFCOMPUTINGANDINFORMATIONSYSTEMS Grippers 0210 nano-technology business Algorithms Computer hardware Biotechnology |
| Zdroj: | IEEE Transactions on NanoBioscience. 18:373-380 |
| ISSN: | 1558-2639 1536-1241 |
| DOI: | 10.1109/tnb.2019.2904009 |
| Popis: | This paper reports a vision-based automated microfluidic system for morphological measurement and size-based sorting of the nematode worm C. elegans . Exceeding the capabilities of conventional worm sorting microfluidic devices purely relying on passive sorting mechanisms, our system is capable of accurate measurement of the worm length/width and active sorting of worms with the desired sizes from a mixture of worms with different body sizes. This function is realized based on the combination of real-time, vision-based worm detection and sizing algorithms and automated on-chip worm manipulation. A double-layer microfluidic device with computer-controlled pneumatic valves is developed for sequential loading, trapping, vision-based sizing, and sorting of single worms. To keep the system operation robust, vision-based algorithms on detecting multi-worm loading and worm sizing failure have also been developed. We conducted sorting experiments on 319 worms and achieved an average sorting speed of 10.4 worms per minute (5.8 s/worm) with an operation success rate of 90.3%. This system will facilitate the worm biology studies where body size measurement and size-based sorting of many worms are needed. |
| Databáze: | OpenAIRE |
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