Intestinal bile acids induce a morphotype switch in vancomycin-resistant enterococcus that facilitates intestinal colonization

Autor: Peter T. McKenney, Eric G. Pamer, Jinyuan Yan, Julien Vaubourgeix, Joao B. Xavier, Daniel Dannaoui, Simone Becattini, Peter J. Larson, Andrew Motzer, Sho Fujisawa, Nina Lampen
Jazyk: angličtina
Rok vydání: 2019
Předmět:
SIGMA-FACTOR
HOST
VRE
Enterococcus faecium
medicine.disease_cause
faecium
Mice
chemistry.chemical_compound
Cecum
0302 clinical medicine
1108 Medical Microbiology
SALTS STRESS-RESPONSE
morphotype
Diplococcus
0303 health sciences
Virulence
Bile acid
SYSTEM WALKR
faecalis
medicine.anatomical_structure
Carrier State
Life Sciences & Biomedicine
0605 Microbiology
Lithocholic acid
Colon
medicine.drug_class
Immunology
bile
Colonisation resistance
Biology
Microbiology
DAPTOMYCIN RESISTANCE
Article
Vancomycin-Resistant Enterococci
Bile Acids and Salts
03 medical and health sciences
Virology
colonization resistance
medicine
microbiota
Animals
Vancomycin-resistant Enterococcus
CHAIN-LENGTH
CELL-SIZE
Gram-Positive Bacterial Infections
030304 developmental biology
Science & Technology
BIOFILM FORMATION
Biofilm
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
bacterial infections and mycoses
chemistry
Enterococcus
Parasitology
030217 neurology & neurosurgery
Zdroj: 705.e5
Cell Host Microbe
Popis: Summary Vancomycin-resistant Enterococcus (VRE) are highly antibiotic-resistant and readily transmissible pathogens that cause severe infections in hospitalized patients. We discovered that lithocholic acid (LCA), a secondary bile acid prevalent in the cecum and colon of mice and humans, impairs separation of growing VRE diplococci, causing the formation of long chains and increased biofilm formation. Divalent cations reversed this LCA-induced switch to chaining and biofilm formation. Experimental evolution in the presence of LCA yielded mutations in the essential two-component kinase yycG/walK and three-component response regulator liaR that locked VRE in diplococcal mode, impaired biofilm formation, and increased susceptibility to the antibiotic daptomycin. These mutant VRE strains were deficient in host colonization because of their inability to compete with intestinal microbiota. This morphotype switch presents a potential non-bactericidal therapeutic target that may help clear VRE from the intestines of dominated patients, as occurs frequently during hematopoietic stem cell transplantation.
Databáze: OpenAIRE
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