The esterase profile of a lipase from Candida cylindracea
| Autor: | L. G. Sparrow, Lothar Elling, M. L. Guglielmino, M. C. Brahimi-Horn |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Tributyrin
Biophysics Triacylglycerol lipase Biochemistry Esterase Chromatography DEAE-Cellulose Substrate Specificity chemistry.chemical_compound Endocrinology Lipase Candida Gel electrophoresis Cetyl palmitate Chromatography biology Isoelectric focusing Hydrophilic interaction chromatography Hydrolysis Esterases chemistry biology.protein Electrophoresis Polyacrylamide Gel Isoelectric Focusing Chromatography Liquid |
| Zdroj: | Biochimica et biophysica acta. 1042(1) |
| ISSN: | 0006-3002 |
| Popis: | A commercial preparation of a lipase produced by Candida cylindracea catalysed the hydrolysis of both long- and short-chain esters of p -nitrophenol. Six major bands of hydrolytic activity to α-naphthyl acetate were detected on polyacrylamide gel electrophoresis and two on isoelectric focusing. The esterase activity fractionated into two major peaks of activity on ion-exchange chromatography and into several peaks of activity on hydrophobic interaction chromatography. These esterase activities showed different substrate specificities to p -nitrophenyl esters, tributyrin and cetyl palmitate. |
| Databáze: | OpenAIRE |
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