Label-free quantitative proteomics analysis of Humulus scandens (Lour.) Merr. leaves treated by an odor compound of Periploca sepium Bunge
Autor: | Li Yan, Zhaoguo Wang, Dinglei Xiang, Sheng Liu, Xue Yang, Shuai Yu, Xiaxia Wang, Zi-yi Wang, Qiuxia Pang |
---|---|
Rok vydání: | 2020 |
Předmět: |
Proteomics
China Proteome Health Toxicology and Mutagenesis Quantitative proteomics 0211 other engineering and technologies Humulus scandens (Lour.) Merr 02 engineering and technology 010501 environmental sciences Humulus Periploca sepium Bunge 01 natural sciences Environmental pollution chemistry.chemical_compound Gene expression GE1-350 Photosynthesis Allelopathy 0105 earth and related environmental sciences 021110 strategic defence & security studies Periploca biology Vanillin Public Health Environmental and Occupational Health General Medicine biology.organism_classification Pollution Chloroplast Environmental sciences Plant Leaves Biochemistry chemistry Odor TD172-193.5 Benzaldehydes 2-Hydroxy-4-methoxy-benzaldehyde Odorants |
Zdroj: | Ecotoxicology and Environmental Safety, Vol 215, Iss, Pp 112131-(2021) |
ISSN: | 1090-2414 |
Popis: | The odor compound from Periploca sepium Bunge, 2-hydroxy-4-methoxy-benzaldehyde (HMB), is an allelochemical agent and is one of the least investigated isomers of vanillin. In this study, we used label-free quantitative proteomics analysis technology to investigate the effect of HMB on the protein expression of Humulus scandens (Lour.) Merr. leaves in July 2019 on Guiyang. A total of 269 proteins of 624 identified proteins were differentially expressed, among which 21.18% of the proteins were up-regulated and 32.71% down-regulated. These proteins were classified into 11 cell components and more than 20% of differentially expressed proteins were located in cell membrane and chloroplast. Functional classification analysis showed that 12 molecular functions were altered upon HMB treatment, and the ratio of catalytic activity was the highest (19.53%). At least 12 biological functions were affected, which involved small molecule metabolic processes, organic substance metabolic processes, gene expression, and photosynthesis. Our data provide resources and insights into the biochemical mechanism by which HMB kills weeds. |
Databáze: | OpenAIRE |
Externí odkaz: |