Immunoglobulin isotype compositions of ABO specific antibodies are dependent on the individual patient blood group and blood group specificity: Results from a healthy donor cohort
Autor: | Nithya Krishnan, Ian Skidmore, David Lowe, Shimon Hussain, Sunil Daga, Prashanth Patel, Robert Higgins, Daniel A. Mitchell, Manjit Kaur Braitch, David Briggs, Andrew Bentall, Daniel Zehnder, Simon Ball |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Male Immunology Blood Donors Group A Flow cytometry ABO Blood-Group System Cohort Studies 03 medical and health sciences Epitopes 0302 clinical medicine Antigen ABO blood group system Immunology and Allergy Medicine Humans medicine.diagnostic_test biology business.industry Reproducibility of Results Flow Cytometry Isotype Immunoglobulin Isotypes Agglutination (biology) 030104 developmental biology Blood Grouping and Crossmatching IgG binding biology.protein Blood Group Antigens Female Antibody business 030215 immunology |
Zdroj: | Journal of immunological methods. 494 |
ISSN: | 1872-7905 |
Popis: | Antibodies specific for the blood group ABO system antigens are of clinical significance and immunological interest. Routine clinical methods typically employ direct or indirect haemagglutination methods to measure IgM and IgG, respectively. We have developed a simple, single tube method to quantify IgM, IgG, and IgA specific for A and B antigens in order to improve accuracy and reproducibility, and to investigate the relationships between ABO group antibody type, and antibody level. Plasma samples from 300 healthy blood donors were studied. Levels of IgM and IgG binding to reagent group A and B red cells were measure by agglutination (HA) and multi-colour flow cytometry (MC-FC). IgA was also measured by MC-FC. Our FC method was found to be significantly more reproducible than HA for the measurement of blood group A and B specific antibodies. We found statistically significant correlations between antibodies measured by GC-HA and MC-FC, but sufficient differences to indicate that these methods are not equivalent. By MC-FC, IgM, IgG and IgA levels and isotope profiles were found to be dependent on both the donor ABO type and the specificity of the antibody. This study demonstrated heterogeneity in the immunoglobulin class profiles of ABO-blood group specific antibodies within the healthy population. Differences in isotype profiles of ABO-blood group specific antibodies may indicate fundamental differences in the immune mechanisms that generate these antibodies. This is likely to be relevant to the clinical situations where management or diagnosis depend on ABO-specific antibody detection and measurement. |
Databáze: | OpenAIRE |
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