PHOTOFRIN II PHOTOSENSITIZATION IS MUTAGENIC AT THE tk LOCUS IN MOUSE L5178Y CELLS
| Autor: | Marian E. Clay, Helen H. Evans, Nancy L. Oleinick, Antonio R. Antunez, Ronald M. Rerko |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Radiation-Sensitizing Agents
endocrine system Light Ethyl methanesulfonate Cell Survival Ratón Mutant Locus (genetics) Biology Thymidine Kinase Biochemistry Mice chemistry.chemical_compound Tumor Cells Cultured Animals Photosensitizer Leukemia L5178 Physical and Theoretical Chemistry Cytotoxicity X-Rays fungi food and beverages General Medicine Molecular biology Hematoporphyrins chemistry Mutagenesis Cell culture Thymidine kinase Dihematoporphyrin Ether |
| Zdroj: | Photochemistry and Photobiology. 55:75-80 |
| ISSN: | 1751-1097 0031-8655 |
| Popis: | — Photosensitization mediated by Photofrin II (PFII) was found to be mutagenic at the heterozygous thymidine kinase (tk) locus in mouse L5178Y lymphoma strains LY-S1 and LY-R16 but not in strain LY-R83 which is hemizygous at the tk locus. After treatments yielding 37% survival, the mutagenicity of photosensitization with PFII in strain LY-S1 was similar to that of other mutagenic agents including x-radiation, ethyl methanesulfonate, and photosensitization with chloroaluminum phthalocyanine (AIPcCI). Although both strain LY-S1 and strain LY-R16 were mutagenized by photosensitization with PFII, only strain LY-S1 was mutagenized by photosensitization with AIPcCI. The non-mutability of strain LY-R83 following photodynamic treatment with either sensitizer may be because of the poor recovery of mutants with intergenic mutations in this TK+/0 hemizygous strain, whereas the non-mutability of strain LY-R16 subjected to photodynamic treatment with AIPcCI may be because LY-R16 cells sustaining mutagenic damage do not survive for reasons other than the loss of an essential gene. |
| Databáze: | OpenAIRE |
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