Interferon stimulated genes as peripheral diagnostic markers of early pregnancy in sheep: a critical assessment
| Autor: | Caroline Eozenou, S. Inghels, B. Grimard, Corinne Giraud-Delville, Olivier Sandra, D. Capo, F. Constant, Lindomar Pinto da Silva, V. Mauffré |
|---|---|
| Přispěvatelé: | Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Université Paris Saclay (COmUE), UE 1298 Unité Commune d'Expérimentation Animale, Institut National de la Recherche Agronomique (INRA), ANR-08-GENM-037 |
| Rok vydání: | 2016 |
| Předmět: |
Myxovirus Resistance Proteins
0301 basic medicine Pregnancy test sheep medicine.medical_specialty Pregnancy Tests Pregnancy Proteins Biology Real-Time Polymerase Chain Reaction SF1-1100 Peripheral blood mononuclear cell Virus 03 medical and health sciences pregnancy diagnosis Pregnancy Interferon Internal medicine Gene expression medicine Animals implantation RNA Messenger Ubiquitins [SDV.BDD]Life Sciences [q-bio]/Development Biology Sheep Domestic 2. Zero hunger early pregnancy Gene Expression Profiling interferon stimulated genes Interferon-stimulated gene Reproducibility of Results [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Animal culture 3. Good health Chemokine CXCL10 Gene expression profiling STAT1 Transcription Factor 030104 developmental biology Real-time polymerase chain reaction Endocrinology ROC Curve Leukocytes Mononuclear Pregnancy Animal Female Animal Science and Zoology Interferons Estrus Synchronization medicine.drug |
| Zdroj: | Animal Animal, Published by Elsevier (since 2021) / Cambridge University Press (until 2020), 2016, 10 (11), pp.1856-1863. ⟨10.1017/S175173111600077X⟩ Animal, Vol 10, Iss 11, Pp 1856-1863 (2016) animal animal, Published by Elsevier (since 2021) / Cambridge University Press (until 2020), 2016, 10 (11), pp.1856-1863. ⟨10.1017/S175173111600077X⟩ |
| ISSN: | 1751-7311 1751-732X |
| DOI: | 10.1017/s175173111600077x |
| Popis: | We investigated the diagnostic reliability of pregnancy detection using changes in interferon stimulated gene (ISG) messenger RNA (mRNA) levels in circulating immune cells in ewes. Two different groups of ewes (an experimental group, experiment 1 and a farm group, experiment 2) were oestrus-synchronized and blood sampled on day 14 (D0=day of insemination in control animals, experiment 1) and day 15 (experiment 2). Real-time PCR were performed to evaluate the abundance of different ISG mRNAs. In the experimental group, peripheral blood mononuclear cells of 29 ewes born and bred in experimental facilities were isolated using a Percoll gradient method. Gene expression for Chemokine (C-X-C motif) ligand 10 (CXCL10), Myxovirus (influenza virus) resistance 1 (MX1) and Signal transducer and activator of transcription 1 (STAT1) mRNA were, respectively, 8.3-fold, 6.1-fold and 2.7-fold higher (P0.10) in CXCL10, STAT1, MX1, Myxovirus (influenza virus) resistance 2 (MX2) and ISG15 ubiquitin-like modifier (ISG15) mRNA expression were found between pregnant and non-pregnant ewes. The ROC curves and the hierarchical classification generated from the real-time PCR data failed to discriminate between pregnant and non-pregnant animals. In this group of animals, our results show a strong variability in ISG expression patterns: 17% of animals identified as non-pregnant by the five tests were in fact pregnant, only 52% of pregnant animals had at least two positive results (two genes above threshold), whereas up to five positive results (five genes above threshold) were needed to avoid misclassification. In conclusion, this study illustrates the high variability in ISG expression levels in immune circulating cells during early pregnancy and, therefore, highlights the limits of using ISG expression levels in blood samples, collected on PAXgene® tubes on farms, for early pregnancy detection in sheep. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|