Evaluation of Ogataea (Hansenula) polymorpha for Hyaluronic Acid Production
| Autor: | Elíbio L. Rech, João Heitor Colombelli Manfrão-Netto, Hugo Costa Paes, Kelly Assis Rodrigues, Cintia M. Coelho, Enzo Bento Queiroz, Nádia Skorupa Parachin |
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| Přispěvatelé: | JOÃO HEITOR COLOMBELLI MANFRÃO-NETTO, UNB, ENZO BENTO QUEIROZ, UNB, KELLY ASSIS RODRIGUES, UNB, CINTIA M. COELHO, UNB, HUGO COSTA PAES, UNB, ELIBIO LEOPOLDO RECH FILHO, Cenargen, NÁDIA SKORUPA PARACHIN, UNB. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Microbiology (medical) Genetic switch Genomic editing Hyaluronic acid Ogataea polymorpha Disaccharide Ogataea polymorpha 1 engineering.material 01 natural sciences Microbiology Article 03 medical and health sciences chemistry.chemical_compound hyaluronic acid 2 010608 biotechnology Virology methylotrophic yeast 3 lcsh:QH301-705.5 030304 developmental biology chemistry.chemical_classification 0303 health sciences promoters 6 biology Strain (chemistry) Methylotrophic yeast Glycosidic bond biology.organism_classification Hyaluronan synthase chemistry Biochemistry lcsh:Biology (General) Streptococcus zooepidemicus genomic editing 4 biology.protein engineering genetic switch 5 Promoters Biopolymer |
| Zdroj: | Microorganisms, Vol 9, Iss 312, p 312 (2021) Microorganisms Volume 9 Issue 2 Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| ISSN: | 2076-2607 |
| Popis: | Hyaluronic acid (HA) is a biopolymer formed by UDP-glucuronic acid and UDP-N-acetyl-glucosamine disaccharide units linked by β-1,4 and β-1,3 glycosidic bonds. It is widely employed in medical and cosmetic procedures. HA is synthesized by hyaluronan synthase (HAS), which catalyzes the precursors’ ligation in the cytosol, elongates the polymer chain, and exports it to the extracellular space. Here, we engineer Ogataea (Hansenula) polymorpha for HA production by inserting the genes encoding UDP-glucose 6-dehydrogenase, for UDP-glucuronic acid production, and HAS. Two microbial HAS, from Streptococcus zooepidemicus (hasAs) and Pasteurella multocida (hasAp), were evaluated separately. Additionally, we assessed a genetic switch using integrases in O. polymorpha to uncouple HA production from growth. Four strains were constructed containing both has genes under the control of different promoters. In the strain containing the genetic switch, HA production was verified by a capsule-like layer around the cells by scanning electron microscopy in the first 24 h of cultivation. For the other strains, the HA was quantified only after 48 h and in an optimized medium, indicating that HA production in O. polymorpha is limited by cultivation conditions. Nevertheless, these results provide a proof-of-principle that O. polymorpha is a suitable host for HA production. |
| Databáze: | OpenAIRE |
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