Reactive Oxygen Species Mediate Nicorandil-induced Metabolic Tolerance to Spinal Cord Injury
| Autor: | Muhammad Aftab, Xianzhong Meng, Christian V. Ghincea, Joseph C. Cleveland, David A. Fullerton, T. Brett Reece, Yuki Ikeno, Michael J. Weyant, Linling Cheng, Gavriel F. Roda |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male Ischemia 030204 cardiovascular system & hematology Pharmacology Neuroprotection 03 medical and health sciences Mice 0302 clinical medicine medicine Animals Nicorandil Spinal cord injury Spinal Cord Injuries chemistry.chemical_classification Neurons Reactive oxygen species business.industry Spinal Cord Ischemia medicine.disease Spinal cord Adenosine Potassium channel Mitochondria Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure 030228 respiratory system chemistry Reperfusion Injury cardiovascular system Surgery Cardiology and Cardiovascular Medicine business Reactive Oxygen Species medicine.drug Signal Transduction |
| Zdroj: | The Annals of thoracic surgery. 112(1) |
| ISSN: | 1552-6259 |
| Popis: | Spinal cord injury remains a devastating complication of thoracoabdominal aortic surgery. We previously demonstrated that pretreatment with nicorandil preserved motor function in a murine spinal cord injury model through mitochondrial adenosine triphosphate-sensitive potassium channel activation. We hypothesized that the neuroprotective effect of nicorandil is mediated by downstream generation of reactive oxygen species.Spinal cord injury was induced by 7 minutes of thoracic aortic cross-clamping in adult male C57BL/6 mice. Five groups were evaluated: ischemic control (n = 19); nicorandil 1.0 mg/kg (n = 17); nicorandil 1.0 mg/kg plus N acetyl L-cysteine (NAC [reactive oxygen species scavenger, n = 18)]) 150 mg/kg; NAC 150 mg/kg (n = 13); and sham (n = 10). Limb motor function and the number of viable neurons within the anterior horn of the spinal cord were evaluated.Mice in the sham group showed no functional deficits after surgery. Compared with ischemic control, motor function was significantly preserved in the nicorandil pretreatment group at every timepoint after ischemia. In the nicorandil plus NAC group, the motor-preserving effect of nicorandil was completely abolished (P.001). Viable neuron quantification showed significant neuron preservation in the nicorandil group (29.± 2.6) compared with the ischemic control group (18.5 ± 2.1, P = .024) and nicorandil plus NAC group (14 ± 8.3, P = .001); no significant difference was observed between the ischemic control group and nicorandil plus NAC group (P = 0.768).Reactive oxygen species generation plays a key role in the nicorandil-induced metabolic tolerance to spinal cord injury. Manipulation of mitochondrial adenosine triphosphate-sensitive potassium channels may lead to improvement in preventing spinal cord injury after thoracoabdominal aortic interventions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|