Characterization ofCandida albicans colony morphological mutants and their hybrids by means of RAPD-PCR, isoenzyme analysis and pathogenicity analysis
| Autor: | Miklós Pesti, L. Emödy, C. Vágvölgyi, A. Novák |
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| Rok vydání: | 2004 |
| Předmět: |
clone (Java method)
Base Sequence Virulence Somatic cell Auxotrophy Genes Fungal Mutant Germ tube General Medicine Biology biology.organism_classification Microbiology Corpus albicans Random Amplified Polymorphic DNA Technique RAPD Isoenzymes Mice Phenotype Candida albicans Mutation Animals Hybridization Genetic DNA Fungal |
| Zdroj: | Folia Microbiologica. 49:527-533 |
| ISSN: | 1874-9356 0015-5632 |
| DOI: | 10.1007/bf02931528 |
| Popis: | A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutants. Out of the isolated 10 clones, 2 stable hybrid clones were chosen and characterized: clone VI. 1M produced rough colonies containing a new, extended cell type (never observed in natural isolates), exhibited unipolar budding, did not form a germ tube, and possessed 12 chromosomal bands. All other features (antifungal and stress sensitivity, adhesion ability, pathogenicity, and isoenzyme and RAPD patterns) were similar to those of mycelial mutant M5. In contrast, the characteristics of clone VI.9S were similar to those of morphological mutant M2. |
| Databáze: | OpenAIRE |
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