Histochemical studies on the depletion of noradrenaline by adrenaline in adrenergic nerves of the rat iris
| Autor: | C. G. Haining, L. R. Murray, Julia L. Heydon |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
medicine.medical_specialty
Sympathetic Nervous System Epinephrine Noradrenaline uptake Iris Pharmaceutical Science Ascorbic Acid In Vitro Techniques Norepinephrine Adrenergic Nerves Internal medicine Cornea medicine Animals Iris (anatomy) Incubation Pharmacology Histocytochemistry Chemistry Temperature Fluorescence Rats Fluorescence intensity Endocrinology medicine.anatomical_structure Microscopy Fluorescence Krebs solution |
| Zdroj: | Journal of Pharmacy and Pharmacology. 21:639-647 |
| ISSN: | 2042-7158 0022-3573 |
| DOI: | 10.1111/j.2042-7158.1969.tb08139.x |
| Popis: | The intensity of fluorescence produced in adrenergic nerves by exposure to formaldehyde under controlled conditions and levels of tissue catecholamines were determined in rat irides. Incubation of irides in Krebs solution containing (-)-adrenaline (0·1–10 μg/ml) led to a temperature-dependent reduction of fluorescence intensity, a reduction in tissue noradrenaline and a gain in tissue adrenaline. The changes in fluorescence were reversed by subsequent incubation of the tissue in the presence of (-)-noradrenaline (0·1–1 μg/ml). A reduction of fluorescence intensity and tissue noradrenaline in the rat iris, followed by recovery over 16 h, was also produced by exposing the cornea to a 1 or 5% solution of (-)-adrenaline. The application of these results to studies on noradrenaline uptake in adrenergic nerves is discussed. |
| Databáze: | OpenAIRE |
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