Noxa upregulation and 5‐gene apoptotic biomarker panel in colorectal cancer
| Autor: | Vivian Kosmidou, Alexander Pintzas, Chrysanthi Aggeli, Salomi Skarmalioraki, Sofia Raftopoulou, George N Zografos, Theodosia Choreftaki, Eirini Kalogerakou, Margarita Vlassi, Kyriakos Anagiotos |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Colorectal cancer Ubiquitin-Protein Ligases Clinical Biochemistry Apoptosis 030204 cardiovascular system & hematology Gene mutation medicine.disease_cause Biochemistry Inhibitor of Apoptosis Proteins Proto-Oncogene Proteins p21(ras) 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation hemic and lymphatic diseases Biomarkers Tumor medicine Humans MCL1 RNA Messenger 030212 general & internal medicine Aged Aged 80 and over business.industry Cancer General Medicine Middle Aged HCT116 Cells medicine.disease Baculoviral IAP Repeat-Containing 3 Protein Up-Regulation Receptors TNF-Related Apoptosis-Inducing Ligand Real-time polymerase chain reaction Proto-Oncogene Proteins c-bcl-2 Cancer research Myeloid Cell Leukemia Sequence 1 Protein Female KRAS Caco-2 Cells Colorectal Neoplasms business HT29 Cells |
| Zdroj: | European Journal of Clinical Investigation. 51 |
| ISSN: | 1365-2362 0014-2972 |
| DOI: | 10.1111/eci.13353 |
| Popis: | BACKGROUND NOXA and MCL1 are involved in the intrinsic pathway of apoptosis, where Noxa selectively binds to MCL1 and prevents it from inhibiting apoptosis. Both factors are considered as potential tumour biomarkers, while MCL1 has attracted interest as target in cancer. The purpose of this study was to investigate the expression of NOXA and MCL1 in 160 CRC tumour samples, to investigate their significance, also in combination with IAPs, DR5 expression and KRAS gene mutations in CRC. MATERIALS AND METHODS Fresh frozen colorectal tissue was obtained from patients undergoing surgery for CRC. Real-time quantitative PCR was performed for the determination of mRNA expression levels. Protein expression was determined immunohistochemically. Differences in the mRNA expression profile were evaluated with the nonparametric Wilcoxon signed ranks test. Statistical analysis was performed with the use of Mann-Whitney U test and receiver-operating characteristic (ROC) curve. RESULTS NOXA was found to be overexpressed in CRC tumours (P |
| Databáze: | OpenAIRE |
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