Amperometric thin film biosensors based on glucose dehydrogenase and Toluidine blue O as catalyst for NADH electrooxidation
| Autor: | Lin Geng, L. Boguslavsky, Z. Xu, Terje Skotheim, I.P. Kovalev, Lo Gorton, V. Laurinavicius, Bengt Persson, S.K. Sahni |
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| Rok vydání: | 1995 |
| Předmět: |
Immobilized enzyme
Chemistry Glucose Dehydrogenases Biomedical Engineering Biophysics Analytical chemistry Glucose 1-Dehydrogenase Biosensing Techniques General Medicine Electrolyte Chronoamperometry Enzymes Immobilized NAD Amperometry Carbon paste electrode Glucose Glucose dehydrogenase Electrochemistry Tolonium Chloride Cyclic voltammetry Oxidation-Reduction Biosensor Biotechnology Nuclear chemistry |
| Zdroj: | Biosensors and Bioelectronics. 10:693-704 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/0956-5663(95)96960-7 |
| Popis: | Amperometric glucose sensors were constructed based on solid graphite electrodes, surface-modified with NAD + dependent glucose dehydrogenase (GDH), Toluidine Blue O (TBO), and protective ionic polymers. The electrocatalytic oxidation of NADH was evaluated from cyclic voltammetry with TBO dissolved, adsorbed, and electrostatically or covalently bound to polymers. The NADH and glucose sensors constructed were investigated and operated at 0 mV vs. Ag/AgCl using single potential step chronoamperometry. The operational stability of the glucose sensors was limited by leakage of NAD + . A glucose sensitivity much higher than carbon paste electrode was found. A sensitivity as high as 25 μA cm −2 mM −1 was achieved. |
| Databáze: | OpenAIRE |
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