Rbf1(RPG-box binding factor), a Transcription Factor Involved in Yeast-hyphal Transition of Candida albicans
| Autor: | Mikio Arisawa, Yuhko Aoki, Miyuki Watanabe, Fumie Yoshihara, Nobuya Ishii |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
biology
Saccharomyces cerevisiae Mutant Nuclear Proteins biology.organism_classification Microbiology DNA-binding protein Corpus albicans Cell biology DNA-Binding Proteins Fungal Proteins Infectious Diseases Membrane protein Candida albicans Signal transduction Transcription factor Transcription Factors |
| Zdroj: | Nippon Ishinkin Gakkai Zasshi. 39:67-71 |
| ISSN: | 1882-0476 0916-4804 |
| DOI: | 10.3314/jjmm.39.67 |
| Popis: | The major fungal pathogen for fungal diseases which have become a major medical problem in the last few years is Candida albicans, which can grow both in yeast and hyphae forms. This ability of C. albicans is thought to contribute to its colonization and dissemination within host tissues. In a recent few years, accompanying the introduction of molecular biological tools into C. albicans organism, several factors involved in the signal transduction pathway for yeast-hyphal transition have been identified. One MAP kinase pathway in C. albicans, similar to that leading to STE12 activation in Saccharomyces cerevisiae, has been reported. C. albicans strains mutant in these genes show retarded filamentous growth on a solid media but no impairment of filamentous growth in mice. These results suggest two scenarios that a kinase signaling cascade plays a part in stimulating the morphological transition in C. albicans, and that there would be another signaling pathway effective in animals. In this latter true hyphal pathway, although some candidate proteins, such as Efg1 (transcription factor), Int1 (integrin-like membrane protein), or Phr1 (pH-regulated membrane protein), have been identified, it is still too early to say that we understand the whole picture of that cascade. We have cloned a C. albicans gene encoding a novel DNA binding protein, Rbf1, that predominantly localizes in the nucleus, and shows transcriptional activation capability. Disruption of the functional RBF1 genes of C. albicans induced the filamentous growth on all solid and liquid media tested, suggesting that Rbf1 might be another candidate for the true hyphal pathway. Relationships with other factors described above, and the target (regulated) genes of Rbf1 is under investigation. |
| Databáze: | OpenAIRE |
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