| Popis: |
USING techniques and supernatant similar to those used to demonstrate spontaneous potentials from explants of chick embryo cerebellum in tissue culture in Kahn tubes1, spontaneous potentials have been demonstrated in explants from the surface of the upper postero-lateral aspects of human adult cerebellum. The explants were thin slices of the full thickness of cerebellar grey matter together with some of the related white matter. The control used was dead chick cerebellar tissue, side by side with the living culture material in the same incubator, with identical amplification and recording apparatus. The living brain tissue gave the same form and magnitude of potentials on any recording channel which was tried. The form of the potentials is shown in Figs. 1 and 2. Fig. 1 shows the typical activity after 12 hr. in culture. This consists of pairs of potentials having a complex wave-form. The first of the potentials in each pair has a magnitude of 75 μV. and the other, which follows after an interval of 12–15 sec., has about one-third of this potential. The interval between the pairs of spikes is about 45 sec. Fig. 2 shows the typical activity after 24 hr. in culture. The pairing of the spikes is much less common, and when it does occur the interval between the two members of the pair is very irregular. The interval between the potentials is more irregular and varies about 20 sec. The potentials at this time have a magnitude of about 20 μV. The culture continued to have spontaneous electrical activity until more than 72 hr. after explantation. No record of spontaneous potentials from adult brain in culture have been found in the literature. Further investigations of these potentials are in progress. |
