Products of Docosahexaenoate Oxidation as Contributors to Photosensitising Properties of Retinal Lipofuscin
| Autor: | Bartosz Rozanowski, Malgorzata Barbara Rozanowska, Anna Pawlak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
retina Docosahexaenoic Acids Light genetic structures photosensitised oxidation retinal pigment epithelium Photochemistry Article Catalysis singlet oxygen Lipofuscin photosensitiser Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Superoxides Humans docosahexaenoate Physical and Theoretical Chemistry Triplet state Molecular Biology lcsh:QH301-705.5 lipofuscin Spectroscopy chemistry.chemical_classification reactive oxygen species Reactive oxygen species Autoxidation Superoxide Singlet oxygen Organic Chemistry Retinal General Medicine Chromophore docosahexaenoic acid Photochemical Processes eye diseases Computer Science Applications 030104 developmental biology chemistry lcsh:Biology (General) lcsh:QD1-999 030221 ophthalmology & optometry sense organs superoxide Oxidation-Reduction |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 7 International Journal of Molecular Sciences, Vol 22, Iss 3525, p 3525 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22073525 |
| Popis: | Retinal lipofuscin which accumulates with age in the retinal pigment epithelium (RPE) is subjected to daily exposures to high fluxes of visible light and exhibits potent photosensitising properties however, the molecules responsible for its photoreactivity remain unknown. Here, we demonstrate that autooxidation of docosahexaenoate (DHE) leads to the formation of products absorbing, in addition to UVB and UVA light, also visible light. The products of DHE oxidation exhibit potent photosensitising properties similar to photosensitising properties of lipofuscin, including generation of an excited triplet state with similar characteristics as the lipofuscin triplet state, and photosensitised formation of singlet oxygen and superoxide. The quantum yields of singlet oxygen and superoxide generation by oxidised DHE photoexcited with visible light are 2.4- and 3.6-fold higher, respectively, than for lipofuscin, which is consistent with the fact that lipofuscin contains some chromophores which do contribute to the absorption of light but not so much to its photosensitising properties. Importantly, the wavelength dependence of photooxidation induced by DHE oxidation products normalised to equal numbers of incident photons is also similar to that of lipofuscin—it steeply increases with decreasing wavelength. Altogether, our results demonstrate that products of DHE oxidation include potent photosensitiser(s) which are likely to contribute to lipofuscin photoreactivity. |
| Databáze: | OpenAIRE |
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