Compact solid-state CMOS single-photon detector array for in vivo NIR fluorescence lifetime oncology measurements
| Autor: | Edoardo Charbon, Krisztian Homicsko, F. Powolny, Elena A. Dubikovskaya, K. Muehlethaler, Jouke Dijkstra, Paulien L. Stegehuis, D. Rimoldi, Harald Homulle, R Sinisi, Du Li, John O. Prior, Claudio Bruschini |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
ocis:(170.3650) Lifetime-based sensing
Materials science Photodetector 01 natural sciences Article RS 030218 nuclear medicine & medical imaging 010309 optics 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Nuclear magnetic resonance In vivo 0103 physical sciences Fluorescence microscope Near-infrared spectroscopy ocis:(170.2520) Fluorescence microscopy Nanosecond Atomic and Molecular Physics and Optics Photon counting ocis:(230.5160) Photodetectors chemistry ocis:(030.5260) Photon counting ocis:(170.3880) Medical and biological imaging Biological imaging Indocyanine green Biotechnology |
| Zdroj: | Biomedical Optics Express, vol. 7, no. 5, pp. 1797-1814 Biomedical Optics Express Biomedical Optics Express, 7(5), 1797-1814 |
| ISSN: | 2156-7085 |
| Popis: | In near infrared fluorescence-guided surgical oncology, it is challenging to distinguish healthy from cancerous tissue. One promising research avenue consists in the analysis of the exogenous fluorophores' lifetime, which are however in the (sub-)nanosecond range. We have integrated a single-photon pixel array, based on standard CMOS SPADs (single-photon avalanche diodes), in a compact, time-gated measurement system, named FluoCam. In vivo measurements were carried out with indocyanine green (ICG)-modified derivatives targeting the alpha(v)beta(3) integrin, initially on a genetically engineered mouse model of melanoma injected with ICG conjugated with tetrameric cyclic pentapeptide (ICG-E[c(RGDfK)(4)]), then on mice carrying tumour xenografts of U87-MG (a human primary glioblastoma cell line) injected with monomeric ICG c (RGDfK). Measurements on tumor, muscle and tail locations allowed us to demonstrate the feasibility of in vivo lifetime measurements with the FluoCam, to determine the characteristic lifetimes (around 500 ps) and subtle lifetime differences between bound and unbound ICG-modified fluorophores (10% level), as well as to estimate the available photon fluxes under realistic conditions. (C) 2016 Optical Society of America |
| Databáze: | OpenAIRE |
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