Members of the Hyposoter didymator Ichnovirus repeat element gene family are differentially expressed in Spodoptera frugiperda
| Autor: | L. Galibert, Janick Rocher, Philippe Fournier, Anne-Nathalie Volkoff, M. Barat-Houari, Gérard Devauchelle, François Cousserans, Pierre Cérutti |
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| Přispěvatelé: | Biologie Intégrative et Virologie des Insectes [Univ. de Montpellier II] (BIVI), Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Saint-Christol (SAINT-CHRISTOL), Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2006 |
| Předmět: |
Gene Expression Regulation
Viral food.ingredient expression génique Genes Viral Transcription Genetic Virologie virus entomopathogène viruses Molecular Sequence Data Wasps Gene Dosage Insect Viruses virus Spodoptera Genome Gene dosage lcsh:Infectious and parasitic diseases HYPOSOTER DIDYMATOR Viral Proteins food Transcription (biology) Virology Animals BIOLOGIE DU DEVELOPPEMENT Gene family lcsh:RC109-216 Amino Acid Sequence Peptide sequence Gene SPODOPTERA FRUGIPERDA Repetitive Sequences Nucleic Acid Genetics biology Research fungi BIOLOGIE MOLECULAIRE biology.organism_classification Molecular biology Infectious Diseases Polydnaviridae Larva Multigene Family [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Ichnovirus |
| Zdroj: | Virology Journal Virology Journal, BioMed Central, 2006, 3, pp.48. ⟨10.1186/1743-422X-3-48⟩ Virology Journal, Vol 3, Iss 1, p 48 (2006) Virology Journal (3), 48. (2006) |
| ISSN: | 1743-422X |
| Popis: | Background The abundance and the conservation of the repeated element (rep) genes in Ichnoviruses genomes suggest that this gene family plays an important role in viral cycles. In the Ichnovirus associated with the wasp Hyposoter didymator, named HdIV, 10 rep genes were identified to date. In this work, we report a relative quantitative transcription study of these HdIV rep genes in several tissues of the lepidopteran host Spodoptera frugiperda as well as in the H. didymator wasps. Results The data obtained in this work indicate that, in the early phases of infection (24 hours), HdIV rep genes each display different levels of transcripts in parasitized 2nd instar or HdIV-injected last instar S. frugiperda larvae. Only one, rep1, is significantly transcribed in female wasps. Transcript levels of the HdIV rep genes were found as not correlated to their copy number in HdIV genome. Our results also show that HdIV rep genes display different tissue specificity, and that they are primarily transcribed in S. frugiperda fat body and cuticular epithelium. Conclusion This work is the first quantitative analysis of transcription of the ichnovirus rep gene family, and the first investigation on a correlation between transcript levels and gene copy numbers in Ichnoviruses. Our data indicate that, despite similar gene copy numbers, not all the members of this gene family are significantly transcribed 24 hours after infection in lepidopteran larvae. Additionally, our data show that, as opposed to other described HdIV genes, rep genes are little transcribed in hemocytes, thus suggesting that they are not directly associated with the disruption of the immune response but rather involved in other physiological alterations of the infected lepidopteran larva. |
| Databáze: | OpenAIRE |
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