Photoswitchable Molecular Communication between Programmable DNA‐Based Artificial Membraneless Organelles

Autor: Qi‐Hong Zhao, Fang‐Hao Cao, Zhen‐Hong Luo, Wilhelm T. S. Huck, Nan‐Nan Deng
Rok vydání: 2022
Předmět:
Zdroj: Angewandte Chemie, 134, 14, pp. 1-5
Angewandte Chemie. International Edition, 61, 1-5
Angewandte Chemie, 134, 1-5
Angewandte Chemie. International Edition, 61, 14, pp. 1-5
ISSN: 1433-7851
1521-3757
Popis: Spatiotemporal organization of distinct biological processes in cytomimetic compartments is a crucial step towards engineering functional artificial cells. Mimicking controlled bi-directional molecular communication inside artificial cells remains a considerable challenge. Here we present photoswitchable molecular transport between programmable membraneless organelle-like DNA coacervates in a synthetic microcompartment. We use droplet microfluidics to fabricate membraneless non-fusing DNA coacervates by liquid-liquid phase separation in a water-in-oil droplet, and employ the interior DNA coacervates as artificial organelles to imitate intracellular communication via photo-regulated uni- and bi-directional transfer of biomolecules. Our results highlight a promising new route to assembly of multicompartment artificial cells with functional networks.
Databáze: OpenAIRE