Protective immunity against infection with Mycoplasma haemofelis
| Autor: | Hicks, Chelsea A E, Willi, Barbara, Riond, Barbara, Novacco, Marilisa, Meli, Marina L, Stokes, Christopher R, Helps, Christopher R, Hofmann-Lehmann, Regina, Tasker, Séverine |
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| Přispěvatelé: | University of Zurich, Tasker, Séverine |
| Rok vydání: | 2015 |
| Předmět: |
Male
10253 Department of Small Animals Enzyme-Linked Immunosorbent Assay 1308 Clinical Biochemistry Cat Diseases Real-Time Polymerase Chain Reaction 2726 Microbiology (medical) Mycoplasma Animals HSP70 Heat-Shock Proteins Mycoplasma Infections Lymphocytes 11434 Center for Clinical Studies 2403 Immunology 630 Agriculture Gene Expression Profiling Flow Cytometry Antibodies Bacterial Bacterial Load Blood Cats 11404 Department of Clinical Diagnostics and Services 2723 Immunology and Allergy Cytokines 570 Life sciences biology Clinical Immunology |
| Zdroj: | Hicks, C A E, Willi, B, Riond, B, Novacco, M, Meli, M L, Stokes, C R, Helps, C R, Hofmann-Lehmann, R & Tasker, S 2015, ' Protective Immunity against Infection with Mycoplasma haemofelis ', Clinical and Vaccine Immunology, vol. 22, no. 1, pp. 108-118 . https://doi.org/10.1128/CVI.00581-14 Clinical and Vaccine Immunology : CVI |
| DOI: | 10.5167/uzh-104080 |
| Popis: | Hemoplasmas are potentially zoonotic mycoplasmal pathogens, which are not consistently cleared by antibiotic therapy. Mycoplasma haemofelis is the most pathogenic feline hemoplasma species. The aim of this study was to determine how cats previously infected with M. haemofelis that had recovered reacted when rechallenged with M. haemofelis and to characterize the immune response following de novo M. haemofelis infection and rechallenge. Five specific-pathogen-free (SPF)-derived naive cats (group A) and five cats that had recovered from M. haemofelis infection (group B) were inoculated subcutaneously with M. haemofelis. Blood M. haemofelis loads were measured by quantitative PCR (qPCR), antibody response to heat shock protein 70 (DnaK) by enzyme-linked immunosorbent assay (ELISA), blood lymphocyte cell subtypes by flow cytometry, and cytokine mRNA levels by quantitative reverse transcriptase PCR. Group A cats all became infected with high bacterial loads and seroconverted, while group B cats were protected from reinfection, thus providing the unique opportunity to study the immunological parameters associated with this protective immune response against M. haemofelis. First, a strong humoral response to DnaK was only observed in group A, demonstrating that an antibody response to DnaK is not important for protective immunity. Second, proinflammatory cytokine interleukin-6 (IL-6) mRNA levels appeared to increase rapidly postinoculation in group B, indicating a possible role in protective immunity. Third, an increase in IL-12p35 and -p40 mRNA and decrease in the Th2/Th1 ratio observed in group A suggest that a Th1-type response is important in primary infection. This is the first study to demonstrate protective immunity against M. haemofelis reinfection, and it provides important information for potential future hemoplasma vaccine design. |
| Databáze: | OpenAIRE |
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