Abundance Ratio-Dependent Proteomic Analysis by Mass Spectrometry
| Autor: | Michael E. Wright, Hookeun Lee, Timothy J. Griffin, Jeffrey A. Ranish, Iryna Chervetsova, Chris M. Lock, Xiao jun Li, Alpesh A. Patel, Ruedi Aebersold, Sharon S. Chen |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Proteomics chemistry.chemical_classification Proteome Chemistry Quantitative proteomics Analytical chemistry Prostatic Neoplasms Peptide Computational biology Tandem mass spectrometry Mass spectrometry Analytical Chemistry Matrix-assisted laser desorption/ionization Abundance (ecology) Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Tumor Cells Cultured Humans Bottom-up proteomics Quantitative analysis (chemistry) |
| Zdroj: | Analytical Chemistry. 75:867-874 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | The goal of quantitative proteomics is to determine the identity and relative quantity of each protein present in two or more complex protein samples. Here we describe a novel approach to quantitative proteomics. It is based on a highly accurate algorithm for the automated quantification of chromatographically fractionated, isotope-coded affinity-tagged peptides and MALDI quadrupole time-of-flight tandem mass spectrometry for their identification. The method is capable of detecting and selectively identifying those proteins within a complex mixture that show a difference in relative abundance. We demonstrate the effectiveness and the versatility of this approach in the analysis of a standard protein mixture, protein expression profiling in a human prostate cancer cell line model, and identification of the specific components of the multiprotein transcriptional machinery in S. cerevisiae. |
| Databáze: | OpenAIRE |
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