Viral aggregation: quantitation and kinetics of the aggregation of poliovirus and reovirus
| Autor: | D G Sharp, R Floyd |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Ecology
Chemistry viruses Poliovirus Kinetics Single particle analysis Hydrogen-Ion Concentration Reoviridae medicine.disease_cause Models Biological Applied Microbiology and Biotechnology Virology Solutions Particle aggregation Colloid medicine Biophysics Adsorption Mammalian orthoreovirus 3 Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 35:1079-1083 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.35.6.1079-1083.1978 |
| Popis: | The aggregation of poliovirus and reovirus was followed in buffers at various pH values by means of a single particle analysis (SPA) test. The SPA test used here was modified from the original test reported earlier to prevent disaggregation of virus clumps from invalidating the results. The modified SPA test demonstrated that the efficiency of aggregation, which is a measure of the percentage of collisions which are effective in producing an aggregate, may vary widely depending on the conditions in which the virus is placed. The modified SPA test was also used to demonstrate that the kinetic features of viral aggregation follow the classical laws of colloid particle aggregation, which in turn are solely dependent upon diffusion of the particles as caused by brownian motion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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