The conformation of a conserved stem-loop structure in ribosomal RNA
| Autor: | Hans A. Heus, van Knippenberg Ph |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Magnetic Resonance Spectroscopy
Base Sequence Stereochemistry 5.8S ribosomal RNA Molecular Sequence Data RNA General Medicine Ribosomal RNA Biology Stem-loop Nucleic Acid Denaturation Molecular biology 18S ribosomal RNA Conserved sequence 5S ribosomal RNA Structural Biology RNA Ribosomal 28S ribosomal RNA Nucleic Acid Conformation Thermodynamics Molecular Biology |
| Zdroj: | Journal of biomolecular structuredynamics. 1(2) |
| ISSN: | 0739-1102 |
| Popis: | The RNA of small ribosomal subunits contains a conserved stem-loop structure near the 3' end. Characteristics for the hairpins are: (a) a nine-basepairs stem: (b) a conserved A-UU-G junction in the stem: (c) a conserved sequence Gm6(2)AM6(2)A sequence in the loop (except yeast mitochondria and mutants from bacteria). We are using UV-optics, micro-calorimetry and 500 MHz-NMR to investigate fragments of about 50 nucleotides cleaved from the 3' ends of small ribosomal subunit RNA's by bacteriocins. Our preliminary conclusions are: (1) Dimethylation of the adenines in the loop destabilizes the hairpin because of an increased stacking; (2) melting of the hairpin starts at the ends as well as in the middle at the A-UU-G junction; (3) basepair substitutions have an unexpectedly large effect on thermal stability. |
| Databáze: | OpenAIRE |
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