Nitric Oxide Mediates Nitrite-Sensing and Acclimation and Triggers a Remodeling of Lipids

Autor: Fabrice Rébeillé, Mariette Bedhomme, Leonardo Magneschi, Laurent Fourage, Juliette Jouhet, Michael Reith, Séverine Collin, Eric Maréchal, Lina-Juana Dolch, Frédéric Laeuffer, Christelle Richard, Josselin Lupette, Guillaume Tourcier, Melissa Conte, Patrick J. McGinn, Khawla Seddiki, Robert S. Richards, Erwan Corre
Přispěvatelé: Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Total Refining Chemicals, UMR 1417 PCV Laboratoire de Physiologie Cellulaire Végétale, Centre National de la Recherche Scientifique (CNRS), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Aquatic and Crop Resource Development, National Research Council of Canada (NRC), National Research Council of Canada’s Algal Carbon Conversion Program, ANR-12-BIME-0005,DiaDomOil,Domestication des diatomées pour la production de biocarburants(2012), ANR-11-BTBR-0008/11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Fédération de recherche de Roscoff (FR2424), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2017
Předmět:
0301 basic medicine
Transcription
Genetic
Physiology
Phaeodactylum
Acclimatization
Plant Science
Aquatic ecosystem
MGDG
chemistry.chemical_compound
NOA
Gene Expression Regulation
Plant
Climate change
Phosphoenolpyruvate dehydrogenase
Plastids
Nitrite
Cell Death
Galactosyltransferases
Pollution
Adaptation
Physiological
Biochemistry
Caspases
Metabolic pathway
Ferredoxins
triacylglycerol
Phosphoenolpyruvate carboxykinase
Intracellular
Nitrite Reductases
Monogalactosyldiacylglycerol
Biology
S-Nitroso-N-Acetylpenicillamine
Nitrate reductase
Arginine
Nitric Oxide
Nitric oxide
03 medical and health sciences
Biosynthesis
Genetics
nitrogen cycle
Signaling and Response
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Nitrogen metabolism
Nitrites
Triglycerides
Diatoms
Aldehydes
Phytoplancton
Galactolipids
Gene Expression Profiling
fungi
Diatom
Nitrite reductase
Lipid Metabolism
Triacylglycerol biosynthesis
030104 developmental biology
chemistry
13. Climate action
Gene expression
S-Nitroso-N-acetylpenicillamine
Zdroj: Plant Physiology
Plant Physiology, American Society of Plant Biologists, 2017, 175 (3), pp.1407-1423. ⟨10.1104/pp.17.01042⟩
Plant Physiology, 2017, 175 (3), pp.1407-1423. ⟨10.1104/pp.17.01042⟩
ISSN: 1532-2548
0032-0889
Popis: International audience; Nitric oxide (NO) is an intermediate of the nitrogen cycle, an industrial pollutant, and a marker of climate change. NO also acts as a gaseous transmitter in a variety of biological processes. The impact of environmental NO needs to be addressed. In diatoms, a dominant phylum in phytoplankton, NO was reported to mediate programmed cell death in response to diatom-derived polyunsaturated aldehydes. Here, using the Phaeodactylum Pt1 strain, 2E,4E-decadienal supplied in the micromolar concentration range led to a nonspecific cell toxicity. We reexamined NO biosynthesis and response in Phaeodactylum NO inhibits cell growth and triggers triacylglycerol (TAG) accumulation. Feeding experiments indicate that NO is not produced from Arg but via conversion of nitrite by the nitrate reductase. Genome-wide transcriptional analysis shows that NO up-regulates the expression of the plastid nitrite reductase and genes involved in the subsequent incorporation of ammonium into amino acids, via both Gln synthesis and Orn-urea pathway. The phosphoenolpyruvate dehydrogenase complex is also up-regulated, leading to the production of acetyl-CoA, which can feed TAG accumulation upon exposure to NO. Transcriptional reprogramming leading to higher TAG content is balanced with a decrease of monogalactosyldiacylglycerol (MGDG) in the plastid via posttranslational inhibition of MGDG synthase enzymatic activity by NO. Intracellular and transient NO emission acts therefore at the basis of a nitrite-sensing and acclimating system, whereas a long exposure to NO can additionally induce a redirection of carbon to neutral lipids and a stress response.
Databáze: OpenAIRE
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