Development of a Reliable Analytical Protocol for the Isolation of Cholesterol Oxidation Products—a Comparison of Different Lipid Extraction and Saponification Methods
| Autor: | Constantinou, Michalis S., Georgiou, Christiana A., Kapnissi‐Christodoulou, Constantina P. |
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| Přispěvatelé: | Kapnissi‐Christodoulou, Constantina P. [0000-0003-3755-1052] |
| Rok vydání: | 2014 |
| Předmět: |
Simultaneous determinations
Cholesterol oxidation products Phase separation Sample preparation Extraction Applied Microbiology and Biotechnology High-performance liquid chromatography Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound Column chromatography Oxidation Solid phase extraction Safety Risk Reliability and Quality Solid-phase extraction Potassium hydroxide Chromatography Saponification Chromatographic analysis Chemistry Hydrolysis Extraction procedure Extraction (chemistry) Liquids Lipid extraction Cholesterol Potassium hydroxide solution High performance liquid chromatographic methods Hexane Safety Research High performance liquid chromatography Food Science |
| Zdroj: | Food Analytical Methods Food Anal.Methods. |
| ISSN: | 1936-976X 1936-9751 |
| DOI: | 10.1007/s12161-014-0034-1 |
| Popis: | The main objectives of this study were (i) the optimization and the validation of a high performance liquid chromatographic method for the simultaneous determination of seven cholesterol oxidation products (COPs), (ii) the development of a reliable analytical protocol for their selective isolation from food samples, and (iii) the application to several foodstuffs. Baseline separation was achieved using a cyano-bonded high-performance liquid chromatography (HPLC) column and a mobile phase of n-hexane/2-propanol/acetone (97:1.5:1.5, v/v). The criteria studied for the choice of the optimum extraction procedure were the selectivity towards COPs and its efficiency in removing matrix interferences. Special attention was given to the stability of 7-keto under saponification conditions. The ideal procedure involved extraction of lipids from the food matrix with chloroform/methanol (2:1, v/v), alkaline hydrolysis with 1 M potassium hydroxide (KOH) solution in 60 % aqueous ethanol and subsequent enrichment of COPs with solid phase extraction using an NH2 column. COPs presented recoveries from 76 to 103 %. © 2014, Springer Science+Business Media New York. 8 6 1499 1507 Cited By :2 |
| Databáze: | OpenAIRE |
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