An All Solid-State Creatinine Biosensor Based on Ammonium-Selective PVC-NH2 Membrane Electrode
| Autor: | Osman Cubuk, Nihat Tinkilic, Vildan Erci, Melda Altikatoglu, Ibrahim Isildak |
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| Přispěvatelé: | Ondokuz Mayıs Üniversitesi, EBYÜ, Fen Edebiyat Fakültesi |
| Rok vydání: | 2013 |
| Předmět: |
Chromatography
Potentiometric titration Atomic and Molecular Physics and Optics chemistry.chemical_compound Membrane chemistry Creatinine Enzyme Sensors Electrode Potentiometry Ionic conductivity Ammonium Creatininase Glutaraldehyde Electrical and Electronic Engineering Biosensor All Solid-State Biosensors Nuclear chemistry |
| Zdroj: | Sensor Letters. 11:585-590 |
| ISSN: | 1546-198X |
| DOI: | 10.1166/sl.2013.2735 |
| Popis: | WOS: 000323236200024 An all solid-state polymeric membrane potentiometric biosensor has been developed for creatinine. The biosensor was based on all solid-state ammonium-selective electrode. The ammonium-selective electrode was prepared by using aminated poly(vinylchloride) together with nonaction and di-cyclohexyl-18-crown-6 as ionic conductivity enhancer. The ammonium-selective electrode exhibited almost a 52 +/- 5 mV slope between 1 x 10(-1) and 8 x 10(-6) M concentration levels of ammonium. Creatininase was immobilized on the surface of the polymeric all solid-state ammonium ion-selective electrode using a simple one step glutaraldehyde covalent attachment method. Potentiometric characteristics of the biosensor were examined in phosphate (pH 6.00, 7.20 and 8.20), Tris-HCl buffer (pH 6.00, 7.20 and 8.20) buffer and physiological model solutions at different creatinine concentrations (1.0 x 10(-1)-1.0 x 10(-6) M). The linear working range and sensitivity of the biosensor were also determined. The creatinine biosensor is highly sensitive and stable in both buffer solutions. Under obtimized conditions, the bioensor showed a linear response between 1 x 10(-1)-8 x 10(-5) M creatinine in both buffers at pH 7.20. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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