T-cell Subset Profile in Kidney Recipients of Extended or Standard Donors
| Autor: | Dávid Péntek, Gergő József Szőllősi, Gergely Ivády, Ildikó Nagy, Eszter Szánthó, Sándor Sipka, Réka P. Szabó, Zsuzsa Hevessy, Bettina Kárai, Sándor Baráth, Balázs Nemes |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Time Factors genetic structures Antigens CD19 Gastroenterology CD19 Flow cytometry Immune system Antigen T-Lymphocyte Subsets Internal medicine medicine Humans Kidney transplantation Aged Retrospective Studies B-Lymphocytes Transplantation Kidney medicine.diagnostic_test biology business.industry Retrospective cohort study Middle Aged medicine.disease Kidney Transplantation Tissue Donors Transplant Recipients Killer Cells Natural Kinetics medicine.anatomical_structure T cell subset biology.protein Female Surgery business |
| Zdroj: | Transplantation Proceedings. 53:1423-1432 |
| ISSN: | 0041-1345 |
| Popis: | Introduction The usage of extended-criteria donors (ECD) became a routinely accepted manner in the last decade. ECD is a potential risk factor for antibody-mediated rejection. Analysis of lymphocyte subsets might be a complementary diagnostic toolkit because there is limited knowledge about this term. Method Between May 12, 2016, and September 4, 2019, a total of 130 patients who had undergone kidney transplant were investigated. Patients were divided in ECD and standard criteria donor (SCD) groups. Blood samples were collected before the operation, then in the first week and after 30, 60, 180, and 365 days. Besides routine laboratory tests, multicolor flow cytometry was performed for lymphocyte subsets. Results ECD grafts were transplanted to older recipients. The number of CD4+ cells increased in the SCDs from the first week to until the end of first month, and then decreased. The number of CD4+ cells decreased from the beginning of the study until the end of first year to 66% of its original value in ECDs. At the first month, the number of CD19+ cells was higher in SCD compared with ECD cases; the number then decreased in both groups. T-regulatory cells had a drop at the first week that lasted until the first month. A bigger increase in SCD and a moderate increase in ECD group were then observed. The kinetics of CD19+ and CD19+ naive cells are similar in the ECD and SCD groups. In the SCD group, cell count decreased in both CD19+ (13%) and CD19+ naive (12%) between third and sixth month. The count of CD19+ cells decreased by 9%, but the count of CD19+ naive cells increased by 11% between the sixth month and first year. Discussion The prolonged postoperative uremic state caused by the poorer initial function, together with an aging immune system, explains the weaker immune response in ECD patients, which may be the cause of the decreased number of memory and regulatory T cells. Older patients with an ECD graft need a tailored, personalized, and less aggressive immunosuppressive treatment. |
| Databáze: | OpenAIRE |
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