Direct Derivatization vs Aqueous Extraction Methods of Fecal Free Fatty Acids for GC–MS Analysis
| Autor: | Trine Kastrup Dalsgaard, Caroline Nebel, Grith Mortensen, Hanne Christine Bertram, Bashar Amer |
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| Rok vydání: | 2015 |
| Předmět: |
Aqueous solution
Chromatography Formic Acid Esters Swine Chemistry Organic Chemistry Extraction (chemistry) Cell Biology Fatty Acids Nonesterified Mass spectrometry Biochemistry Gas Chromatography-Mass Spectrometry Feces chemistry.chemical_compound Reagent Solvents Animals Metabolomics Ethyl chloroformate Extraction methods Gas chromatography–mass spectrometry Derivatization |
| Zdroj: | Lipids. 50:681-689 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/s11745-015-4029-5 |
| Popis: | A comprehensive and accurate determination of free fatty acids (FFA) is required for fecal metabolomic investigations. The present study compares three aqueous extraction methods (1) ULTRA-TURRAX(®), (2) whirl mixing and (3) basic ULTRA-TURRAX extraction of fecal FFA with a direct derivatization approach using ethyl chloroformate as the derivatization reagent before determination by gas chromatography-mass spectrometry. The direct derivatization method resulted in significantly higher estimations (P 0.01) of short- and long-chain fatty acids than was the case when applying the aqueous extraction methods using ULTRA-TURRAX, whirl mixing, or basic ULTRA-TURRAX extraction before the derivatization step. Thus, avoiding an aqueous extraction before derivatization reduces the loss of volatile short-chain FFA and the less water-soluble long-chain FFA. |
| Databáze: | OpenAIRE |
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