Development of an ultra-fast liquid chromatography–tandem mass spectrometry method for simultaneous determination of seven flavonoids in rat plasma: Application to a comparative pharmacokinetic investigation of Ginkgo biloba extract and single pure ginkgo flavonoids after oral administration
| Autor: | Qing Li, Tianyang Wang, Ziyue Yuan, Huarong Xu, Huiping Hou, Ran Liu, Pei Li, Kaishun Bi, Jie Xiao |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Clinical Biochemistry Ethyl acetate Administration Oral Sensitivity and Specificity 01 natural sciences Biochemistry Analytical Chemistry Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Animals Chromatography High Pressure Liquid Isorhamnetin Flavonoids Chromatography biology Plant Extracts Ginkgo biloba Ginkgo 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine biology.organism_classification Diosmetin Rats 0104 chemical sciences 030104 developmental biology chemistry Linear Models Kaempferol Luteolin |
| Zdroj: | Journal of Chromatography B. 1060:173-181 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2017.05.021 |
| Popis: | For deeper pharmacokinetic investigation and further curative application of ginkgo flavonoids, a delicate, efficient and precise UFLC-MS/MS technique for synchronous quantitation of seven flavonoids, apigenin, luteolin, naringenin, quercetin, diosmetin, kaempferol and isorhamnetin in rat plasma has been established. After mixing with the internal standard (IS) linarin, bio-samples were pretreated via ethyl acetate for liquid-liquid extraction, then isolated at 0.2ml/min flow rate on a Venusil MP C18 chromatographic column (100mm×2.1mm, 3μm) by means of gradient elution. 0.1% formic acid-water and methanol system was used as the mobile phase. Mass spectrometric inspection was conducted on a 4000Q UFLC-MS/MS system with turbo ion spray source in patterns of negative ion and multiple reaction-monitoring (MRM). All calibration curves proved favorable linearity (R2≥0.9918) in linear ranges. Intra-day and inter-day precisions didn't exceed 14.0% for all the analytes, and the accuracy was within 6.9%. Extraction recoveries of analytes and IS were less than ±15.0% of nominal concentrations. This method has been under thorough and firm verification for a comparative pharmacokinetic research after gavage between Ginkgo biloba extract and single pure ginkgo flavonoids. The results demonstrated that there're evident pharmacokinetic discrepancies, and possible structural influences were innovatively proposed. Generally, substitution with 3-hydroxylation, a double bond in ring C, ring B methoxylation often confer longer onset period. The existence of ring B catechol group gives rise to faster clearance. |
| Databáze: | OpenAIRE |
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