Tissue-type plasminogen activator-mediated activation of plasminogen on the surface of group A, C, and G streptococci
| Autor: | Olli Saksela, Pentti Kuusela, M Ullberg, G Kronvall |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Plasmin
Proteolysis Immunology Biology In Vitro Techniques Microbiology Tissue plasminogen activator 03 medical and health sciences Aprotinin Zymogen Plasminogen Inactivators medicine Humans Fibrinolysin 030304 developmental biology chemistry.chemical_classification 0303 health sciences medicine.diagnostic_test 030306 microbiology Streptococcus Plasminogen Infectious Diseases Enzyme Biochemistry chemistry Tissue Plasminogen Activator Parasitology Electrophoresis Polyacrylamide Gel Plasminogen activator medicine.drug Research Article |
| Popis: | The interaction of Glu-plasminogen with group A, C, and G streptococci and subsequent formation of surface-associated plasminogen by tissue-type plasminogen activator (t-PA) were studied. Binding of 125I-Glu-plasminogen to streptococci greatly facilitated its activation to 125I-Glu-plasmin by exogenous t-PA, whereas activation in the absence of bacteria took place only slowly. Glu-plasmin formed on the streptococcal surface was further converted to the Lys form. Similar activation and modification took place also in the presence of plasminogen-depleted plasma, containing functional t-PA and plasmin inhibitors, indicating that the surface-associated enzymes were protected against these inhibitors. Lys-plasminogen was 10- to 30-fold more potent than Glu-plasminogen or Glu-plasmin in inhibiting the binding of 125I-Glu-plasminogen to streptococci. This indicated a higher affinity of the Lys form towards plasminogen-binding molecule(s) on the streptococcal surface. The surface-associated plasmin was also enzymically active as judged by digestion of chromogenic substrate S-2251. Surface-associated plasmin activity was observed only when the incubations were carried out in the presence of t-PA and Glu-plasminogen or human plasma as the source of plasminogen. Under these conditions, soluble enzymatic activity was also recovered in the supernatant of group A streptococci. This favors the idea that plasmin can be released from the bacterial surface. The findings provide a mechanism for streptococci to adopt proteolytic activity by binding a host-derived enzyme zymogen on their surface, where the subsequent activation then takes place. The results suggest a role for surface-associated plasmin activity in tissue tropism and tissue invasiveness of streptococci. |
| Databáze: | OpenAIRE |
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