In search of tetraploid wheat accessions reduced in celiac disease-related gluten epitopes
| Autor: | Jean-Claude Dusautoir, L.J.W.J. Gilissen, Hetty C. van den Broeck, Xavier Lacaze, Marinus J. M. Smulders, Chen Hongbing, Ingrid M. van der Meer |
|---|---|
| Přispěvatelé: | van den Broeck, Hetty, Wageningen University and Research Centre [Wageningen] (WUR), UMR 1097 Diversité et Adaptation des Plantes Cultivées, Institut National de la Recherche Agronomique (INRA)-Génétique et amélioration des plantes (G.A.P.)-Diversité et Adaptation des Plantes Cultivées (DIA-PC), Domaine expérimental de Melgueil (MONTP MELGUEIL UE), Institut National de la Recherche Agronomique (INRA), Wageningen University and Research [Wageningen] (WUR), Diversité et adaptation des plantes cultivées (UMR DIAPC), Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences bread wheat Ingénierie des aliments Disease Subspecies 01 natural sciences Epitope Epitopes [SDV.IDA]Life Sciences [q-bio]/Food engineering [SDV.BDD]Life Sciences [q-bio]/Development Biology Netherlands Plant Proteins 2. Zero hunger chemistry.chemical_classification 0303 health sciences Vegetal Biology Biologie du développement protéine de gluten food and beverages Development Biology Agricultural sciences storage proteins Alimentation et Nutrition blé tétraploïde France complex Biotechnology maladie coeliaque Glutens durum-wheat Immunoblotting blé hexaploïde Médecine humaine et pathologie Biology subunit genes PRI BIOS Applied Genomics & Proteomics polyploid wheat 03 medical and health sciences domestication pâte alimentaire Botany Humans Storage protein [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Food engineering Food and Nutrition Domestication Molecular Biology maladie humaine 030304 developmental biology Chemotype Plant Extracts triticum Gluten Tetraploidy Celiac Disease chemistry blé dur biology.protein gliadin peptides Human health and pathology Gliadin [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Biologie végétale Sciences agricoles 010606 plant biology & botany |
| Zdroj: | Molecular BioSystems 11 (6), 2206-2213. (2010) Molecular BioSystems, 6(11), 2206-2213 Molecular BioSystems 6 (2010) 11 Molecular BioSystems Molecular BioSystems, Royal Society of Chemistry, 2010, 6 (11), pp.2206-2213. ⟨10.1039/c0mb00046a⟩ |
| ISSN: | 1742-206X 1742-2051 |
| DOI: | 10.1039/c0mb00046a |
| Popis: | Tetraploid wheat (durum wheat) is mainly used for the preparation of pasta. As a result of breeding, thousands of tetraploid wheat varieties exist, but also tetraploid landraces are still maintained and used for local food preparations. Gluten proteins present in wheat can induce celiac disease, a T-cell mediated auto-immune disorder, in genetically predisposed individuals after ingestion. Compared to hexaploid wheat, tetraploid wheat might be reduced in T-cell stimulatory epitopes that cause celiac disease because of the absence of the D-genome. We tested gluten protein extracts from 103 tetraploid wheat accessions (obtained from the Dutch CGN genebank and from the French INRA collection) including landraces, old, modern, and domesticated accessions of various tetraploid species and subspecies from many geographic origins. Those accessions were typed for their level of T-cell stimulatory epitopes by immunoblotting with monoclonal antibodies against the a-gliadin epitopes Glia-alpha 9 and Glia-alpha 20. In the first selection, we found 8 CGN and 6 INRA accessions with reduced epitope staining. Fourteen of the 57 CGN accessions turned out to be mixed with hexaploid wheat, and 5 out of the 8 selected CGN accessions were mixtures of two or more different gluten protein chemotypes. Based on single seed analysis, lines from two CGN accessions and one INRA accession were obtained with significantly reduced levels of Glia-alpha 9 and Glia-alpha 20 epitopes. These lines will be further tested for industrial quality and may contribute to the development of safer foods for celiac patients. |
| Databáze: | OpenAIRE |
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