Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides
| Autor: | German A. Roth, Aldo Alejandro Vilcaes, Gustavo A. Nores, Samanta C. Funes, Romina Comín, Ana Lis Moyano, Fernando J. Irazoqui |
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| Rok vydání: | 2012 |
| Předmět: |
Brain Chemistry
education.field_of_study biology Chemistry Population Biological membrane G(M1) Ganglioside Guillain-Barre Syndrome Biochemistry Molecular biology Neuritis Autoimmune Experimental Immunoglobulin G Rats Affinity chromatography Immunization Antigen Gangliosides biology.protein Animals Cattle Rabbits Antibody education Immunostaining |
| Zdroj: | Glycobiology. 22(12) |
| ISSN: | 1460-2423 |
| Popis: | Immunization of rabbits with bovine brain gangliosides induced an experimental neuropathy, with clinical signs resembling Guillain-Barre syndrome. All the immunized animals developed immunoglobulin G immunoreactivity to GM1 ganglioside. In a few (4 of 27) animals, an additional anti-ganglioside antibody population showing an unusual binding behavior was detected. Enzyme-linked immunosorbent assay and thin-layer chromatography immunostaining analyses showed that the binding of these unusual antibodies required the presence of two co-localized gangliosides. Maximal interaction was observed to a mixture of GM1 and GD1b, but the antibodies also showed "density-dependent" binding to GD1b. The antibodies were purified by affinity chromatography and displayed the ability to target antigens in biological membranes (rat synaptosomes). |
| Databáze: | OpenAIRE |
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