Tannic acid- and thiocarbohydrazide-mediated osmium tetroxide binding in the preparation of human leucocytes for SEM observation
| Autor: | E. D. Lalague, S. Haviernick, C. Derghazarian, L. Pelletier, M.-R. Corvellec, G.H. Cousineau |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
inorganic chemicals
endocrine system Histology Osmium Tetroxide Scanning electron microscope Pathology and Forensic Medicine Metal Fixatives chemistry.chemical_compound Critical point drying Tannic acid Leukocytes medicine Humans Organic chemistry Dehydration Thiocarbohydrazide Microvilli medicine.disease Hydrolyzable Tannins Hydrazines Osmium tetroxide chemistry visual_art Microscopy Electron Scanning visual_art.visual_art_medium Glutaraldehyde Nuclear chemistry |
| Zdroj: | Journal of Microscopy. 132:229-233 |
| ISSN: | 0022-2720 |
| DOI: | 10.1111/j.1365-2818.1983.tb04277.x |
| Popis: | SUMMARY Normal human leucocytes, successively treated with glutaraldehyde-tannic acid-osmium tetroxide-thiocarbohydrazide-osmium tetroxide-thiocarbohydrazide-osmium tetroxide, were prepared for scanning electron microscopy observation. These cells produced well-contrasted, non-charging scanning images compatible with metal-evaporated material. Further, the mononuclear and polymorphonuclear cells resisted shrinkage during dehydration and critical point drying, thus allowing much improved images at high magnification than those covered with evaporated metal. In all cases at least a second thiocarbohydrazide-osmium tetroxide treatment could not be avoided. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text