Hemagglutination assay for human serum fibronectin
| Autor: | Bălună R, Barac D, Tarnavschi R, Belaşcu I |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Erythrocytes
food.ingredient Hemagglutination Microgram Immunology Gelatin Epitope Hemagglutination tests Epitopes food Animals Humans Immunology and Allergy Medicine Sheep Hemagglutination assay biology business.industry Hemagglutination Tests Fibronectins Molecular biology Fibronectin biology.protein business |
| Zdroj: | Journal of Immunological Methods. 79:65-70 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(85)90392-8 |
| Popis: | A hemagglutination assay for human serum fibronectin (Fn) is described. The assay is based on the capacity of Fn to bind to gelatin. Purified and human serum Fn agglutinate chromic chloride treated sheep red cells coated with gelatin in a quantitative and reproducible manner. The lowest Fn concentration giving positive hemagglutination was found to be 7 micrograms/ml, a sensitivity making it suitable for measurement of Fn in both normal and pathologic sera. The assay is simple and rapid and does not require specialized equipment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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