Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF
| Autor: | Katerina Macounova, Paul Yager, Catherine R. Cabrera |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Chromatography
Electrolysis of water biology Isoelectric focusing Chemistry Analytical chemistry Proteins Serum Albumin Bovine Fractionation Analytical Chemistry Volumetric flow rate Transverse plane Ionic strength Electrode biology.protein Animals Cattle Gold Bovine serum albumin Isoelectric Focusing Electrodes Palladium |
| Zdroj: | Analytical chemistry. 73(7) |
| ISSN: | 0003-2700 |
| Popis: | The use of microfluidic channels formed by two electrodes made of gold or palladium to perform transverse isoelectric focusing (IEF) is presented as a means for continuous concentration and fractionation of proteins. The microchannels were 40 mm long with an electrode gap of 1.27 mm and a depth of 0.354 mm. The properties of pH gradients formed as a result of the electrolysis of water were influenced by variation of parameters such as the initial pH, ionic strength, and flow rate. Transverse IEF in pressure-driven flow is demonstrated using bovine serum albumin in a single ampholyte buffer as well as in multiple-component buffers. Experimental results of protein focusing compare well to predictions of a mathematical model. Optimal conditions for efficient continuous fractionation of a protein mixture are summarized and discussed. |
| Databáze: | OpenAIRE |
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