Chronic Constriction Injury of the Infraorbital Nerve in the Rat using modified syringe needle
| Autor: | Robert W. Gear, Luc Jasmin, Melanie Kernisant, Jean-Philippe Vit, Peter T. Ohara |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Orofacial pain Time Factors medicine.medical_treatment Article Statistics Nonparametric Rats Sprague-Dawley Infraorbital nerve Trigeminal ganglion Maxillary Nerve Medicine Animals Ligation Activating Transcription Factor 3 CD11b Antigen business.industry General Neuroscience Syringes Spinal trigeminal nucleus Chronic pain Nerve injury Microsurgery medicine.disease Cranial Nerve Diseases Surgery Rats Disease Models Animal medicine.anatomical_structure Needles Anesthesia Face medicine.symptom business Immunostaining |
| Popis: | Here we report a method for performing a chronic constriction injury (CCI) of the infraorbital nerve (ION) in the rat as a component of a chronic pain model. The surgical approach to the ION is described together with the use of a modified dental syringe needle that simplifies placing two chromic gut ligatures around the ION. This method makes the surgical procedure easier, the nerve injury more consistent across animals and reduces secondary damage to the ION and surrounding tissue. Pain behavior testing together with immunostaining for markers of nerve injury in the spinal trigeminal nucleus show the suitability of this procedure as a model of orofacial pain. |
| Databáze: | OpenAIRE |
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