Vascular endothelial growth inhibitor 174 and its functional domains inhibit epithelial-mesenchymal transition in renal cell carcinoma cells in vitro
| Autor: | Xiaohu Deng, Kan Gong, Yong Yang, Baoan Hong, Ning Zhang, Shayiremu Duoerkun, Changhua Zhou, Qing Li, Siqi Chen, Wenyong Lian, Xin Du |
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| Rok vydání: | 2017 |
| Předmět: |
Tumor Necrosis Factor Ligand Superfamily Member 15
0301 basic medicine Epithelial-Mesenchymal Transition Cell Survival Cell Vimentin Biology Vascular endothelial growth inhibitor 03 medical and health sciences 0302 clinical medicine Protein Domains Cell Line Tumor Genetics medicine Humans Protein Isoforms Epithelial–mesenchymal transition Carcinoma Renal Cell General Medicine Transfection Cell cycle Kidney Neoplasms Up-Regulation Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Cancer research biology.protein A431 cells |
| Zdroj: | International Journal of Molecular Medicine. 40:569-575 |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | The present study was carried out to investigate the effects of vascular endothelial growth inhibitor 174 (VEGI174) and its functional domains (V7 and V8) on epithelial‑mesenchymal transition (EMT) in renal cell carcinoma (RCC) cells in vitro. The RCC cell lines A498 and 786‑O were used in this study. Based on our preliminary study, we selected full‑length VEGI174 and its functional domains (V7 and V8) as the target genes in this study. Plasmids containing VEGI174, V7 or V8 transgenes were constructed and transfected into A498 and 786‑O cell lines. Cytological activity was tested during cell culture. Quantitative PCR and western blot analysis were performed to determine the expression levels of EMT markers (E‑cadherin, vimentin, β‑catenin and Slug). Overexpression of VEGI174, V7 or V8 did not have a significant influence on cell viability (P>0.05). The mRNA level of E‑cadherin was significantly upregulated, while that of vimentin was downregulated in A498VEGIexp, A498V7exp, A498V8exp, 786‑OVEGIexp, 786‑OV7exp and 786‑OV8exp cells compared with the cells containing the empty plasmid controls (P |
| Databáze: | OpenAIRE |
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