Nitric Oxide Exposure Diverts Neural Stem Cell Fate from Neurogenesis Towards Astrogliogenesis
Autor: | Ruxandra Covacu, Mikael Svensson, Lou Brundin, Alexandre I. Danilov, Bo Sonnich Rasmussen, Morten C. Moe, Tomas Olsson, Katarina Hallén, Anna Lobell, Clas B. Johansson |
---|---|
Rok vydání: | 2006 |
Předmět: |
Down-Regulation
Gene Expression Proneural genes Biology Nitric Oxide Nitric oxide chemistry.chemical_compound Glial Fibrillary Acidic Protein Animals Cell Lineage Cells Cultured Gliogenesis Neurons Cell Death Stem Cells Regeneration (biology) Neurogenesis Cell Differentiation Cell Biology Neural stem cell Rats Cell biology Oxygen Neuroepithelial cell chemistry Astrocytes Immunology STAT protein Molecular Medicine Female Nitroso Compounds Developmental Biology |
Zdroj: | STEM CELLS. 24:2792-2800 |
ISSN: | 1549-4918 1066-5099 |
DOI: | 10.1634/stemcells.2005-0640 |
Popis: | Regeneration of cells in the central nervous system is a process that might be affected during neurological disease and trauma. Because nitric oxide (NO) and its derivatives are powerful mediators in the inflammatory cascade, we have investigated the effects of pathophysiological concentrations of NO on neurogenesis, gliogenesis, and the expression of proneural genes in primary adult neural stem cell cultures. After exposure to NO, neurogenesis was downregulated, and this corresponded to decreased expression of the proneural gene neurogenin-2 and beta-III-tubulin. The decreased ability to generate neurons was also found to be transmitted to the progeny of the cells. NO exposure was instead beneficial for astroglial differentiation, which was confirmed by increased activation of the Janus tyrosine kinase/signal transducer and activator of transcription transduction pathway. Our findings reveal a new role for NO during neuroinflammatory conditions, whereby its proastroglial fate-determining effect on neural stem cells might directly influence the neuroregenerative process. |
Databáze: | OpenAIRE |
Externí odkaz: |