Evidence for GC preference by monocot Dicer-like proteins
| Autor: | Hui Wang, M. L. Edwards, Thien Ho, Ian Cooper, Tamas Dalmay, Rachel L. Rusholme Pilcher |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Ribonuclease III
DNA Plant Molecular Sequence Data Biophysics Biology Biochemistry Genome Virus Evolution Molecular microRNA RNA Small Interfering Dactylis Molecular Biology Gene Plant Proteins Genetics Base Composition Base Sequence Potyvirus food and beverages RNA Cell Biology biology.organism_classification biology.protein GC-content Dicer |
| Zdroj: | Biochemical and biophysical research communications. 368(2) |
| ISSN: | 1090-2104 |
| Popis: | Dicot Dicer-like (DCL) enzymes operate preferably on GC rich regions when producing small interfering (si)RNA and micro (mi)RNA. This GC bias, however, is not generic in monocot miRNA productions. From wild Dactylis glomerata naturally infected by Cocksfoot streak potyvirus (CSV), CSV-siRNAs had a greater GC% than the virus genome, indicating that GC rich regions were also preferred by the grass DCLs. This supports the notion that GC preference is an ancient feature for plant DCLs, and suggests that monocot miRNA genes might have evolved to a high GC% resulting in GC bias being not detectable during mature miRNA production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect