Oryzalin as an Efficient Agent for Chromosome Doubling of Haploid Apple Shoots in vitro
| Autor: | F. R. Fillon, Y. Lespinasse, L. Bouvier |
|---|---|
| Přispěvatelé: | Unité mixte de recherche génétique et horticulture Genhort, Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-Institut National d'Horticulture, ProdInra, Migration |
| Rok vydání: | 1994 |
| Předmět: |
0106 biological sciences
Outbreeding depression Rosaceae Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Botany Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Colchicine [SDV.BV] Life Sciences [q-bio]/Vegetal Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences fungi Chromosome Oryzalin biology.organism_classification chemistry Shoot Antimitotic Agent Ploidy Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Breeding Plant Breeding, Wiley, 1994, 113, pp.343-346 |
| ISSN: | 1439-0523 0179-9541 |
| DOI: | 10.1111/j.1439-0523.1994.tb00748.x |
| Popis: | In an outbreeding species such as apple, haploid plants may be especially useful in breeding programmes for the production of homozygous material. However, methods must be available to induce chromosome doubling in the haploid plants. Two antimitotic agents, colchicine and oryzalin, were compared as regards their efficiency in inducing chromosome doubling of in vitro haploid apple shoots. Three colchicine levels (0.025, 0.25 and 1.25 mM) and three oryzalin levels (5, 15 and 30 μM) were evaluated. Three techniques were also used and compared. Survival rate and chromosome counts were determined. Differences were observed between the two antimitotic agents and between the three techniques. This study demonstrates that oryzalin could be a better choice than colchicine for chromosome doubling on haploid apple shoots in vitro. |
| Databáze: | OpenAIRE |
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