Effect of solvent polarity on the Ultrasound Assisted extraction and antioxidant activity of phenolic compounds from habanero pepper leaves (Capsicum chinense) and its identification by UPLC-PDA-ESI-MS/MS

Autor: M.A. Lizardi-Jiménez, Ulises García-Cruz, Emanuel Herrera-Pool, Juan Carlos Cuevas-Bernardino, Soledad Cecilia Pech-Cohuo, Ana Ramos-Díaz, Teresa Ayora-Talavera, Neith Pacheco
Rok vydání: 2021
Předmět:
Acoustics and Ultrasonics
DPPH
Review
02 engineering and technology
Eq CA
Caffeic acid equivalents
Chemical Fractionation
01 natural sciences
Antioxidants
80% MeOH
80% Methanol
chemistry.chemical_compound
Tandem Mass Spectrometry
UPLC
Ultra-high performance liquid chromatography
Chemical Engineering (miscellaneous)
GAE
Gallic acid equivalent
Chromatography
High Pressure Liquid
ABTS
80% Ace
80% Acetone
biology
ε
dielectric constant
021001 nanoscience & nanotechnology
Diosmetin
20% MeOH
20% Methanol
Chemistry
Ultrasonic Waves
PDA
Photodiode detector array
UAE
Apigenin
Hx
Hexane
Capsicum
0210 nano-technology
UAE
Ultrasound-Assisted extraction
Spectrometry
Mass
Electrospray Ionization
QC221-246
W
Water
Ace
Acetone
010402 general chemistry
Eq A
Apigenin equivalent
Solvent polarity
50% MeOH
50% Methanol
Inorganic Chemistry
Phenols
Pepper
AcOEt
Ethyl acetate
Capsicum chinense leaves
Environmental Chemistry
Dielectric constant
Radiology
Nuclear Medicine and imaging
1% AcOH
Acetic acid
QD1-999
CE
Conventional extraction
ComputingMethodologies_COMPUTERGRAPHICS
Chromatography
Mass spectrometry
HT
Proton transfer
SET
Simple electron transfer
Organic Chemistry
Extraction (chemistry)
Acoustics. Sound
TPC
Total phenolic content
Dry wt
Dry weight
biology.organism_classification
Phenolic compounds
0104 chemical sciences
Capsicum chinense
MeOH
Methanol
Plant Leaves
MS
mass spectrometry
chemistry
Eq L
Luteolin equivalents
Solvents
Luteolin
Eq Q
Quercetin equivalents
Zdroj: Ultrasonics Sonochemistry
Ultrasonics Sonochemistry, Vol 76, Iss, Pp 105658-(2021)
ISSN: 1350-4177
DOI: 10.1016/j.ultsonch.2021.105658
Popis: Graphical abstract
Highlights • Ultrasound is a fast method for phenolic compounds recovery from C. chinense leaves. • Solvent polarity has a strong effect on the recovery of phenolic compounds. • Dielectric constant was propose to predict the most appropriate solvent for phenolic extraction. • Antioxidant activity correlates with total phenolic content of C. chinense leaves extracts. • Identification of phenolic compounds was performed by UPLC-PDA-ESI-MS/MS.
Phenolic compounds are secondary metabolites involved in plant adaptation processes. The development of extraction procedures, quantification, and identification of this compounds in habanero pepper (Capsicum chinense) leaves can provide information about their accumulation and possible biological function. The main objective of this work was to study the effect of the UAE method and the polarity of different extraction solvents on the recovery of phenolic compounds from C. chinense leaves. Quantification of the total phenolic content (TPC), antioxidant activity (AA) by ABTS+ and DPPH radical inhibition methods, and the relation between the dielectric constant (ε) as polarity parameter of the solvents and TPC using Weibull and Gaussian distribution models was analyzed. The major phenolic compounds in C. chinense leaves extracts were identified and quantified by UPLC-PDA-ESI-MS/MS. The highest recovery of TPC (24.39 ± 2.41 mg GAE g−1 dry wt) was obtained using MeOH (50%) by UAE method. Correlations between TPC and AA of 0.89 and 0.91 were found for both radical inhibition methods (ABTS+ and DPPH). The Weibull and Gaussian models showed high regression values (0.93 to 0.95) suggesting that the highest phenolic compounds recovery is obtained using solvents with “ε” values between 35 and 52 by UAE. The major compounds were identified as N-caffeoyl putrescine, apigenin, luteolin and diosmetin derivatives. The models presented are proposed as a useful tool to predict the appropriate solvent composition for the extraction of phenolic compounds from C. chinense leaves by UAE based on the “ε” of the solvents for future metabolomic studies.
Databáze: OpenAIRE
Externí odkaz: