Matrix Metalloproteinase-14 Deficiency in Bone Marrow–Derived Cells Promotes Collagen Accumulation in Mouse Atherosclerotic Plaques
| Autor: | Peter Libby, Fabrice Schneider, James P. Canner, Norbert Gerdes, Sai Man Timothy Tang, Guo-Ping Shi, Galina K. Sukhova, Masanori Aikawa, Suneel S. Apte |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Pathology Myocytes Smooth Muscle Neovascularization Physiologic Bone Marrow Cells Matrix metalloproteinase Mice Mice Congenic Cell Movement In vivo Physiology (medical) Internal medicine Matrix Metalloproteinase 13 Matrix Metalloproteinase 14 medicine Animals Myocyte Macrophage Aorta Bone Marrow Transplantation Mice Knockout Enzyme Precursors Tumor Necrosis Factor-alpha business.industry Macrophages Atherosclerosis Extracellular Matrix Enzyme Activation Mice Inbred C57BL Cholesterol medicine.anatomical_structure Endocrinology Receptors LDL Radiation Chimera Diet Atherogenic MMP14 Interstitial collagenase Collagen Bone marrow Cardiology and Cardiovascular Medicine business Lipoprotein |
| Zdroj: | Circulation. 117:931-939 |
| ISSN: | 1524-4539 0009-7322 |
| Popis: | Background— Interstitial collagen plays a crucial structural role in arteries. Although in vitro results suggest collagenase activity for membrane-bound matrix metalloproteinase type 1 (MMP-14), in vivo evidence for such a function in atherosclerosis remains scant. Methods and Results— Because Mmp14 −/− mice die by 3 weeks of age, this study used lethally irradiated low-density lipoprotein receptor–deficient mice reconstituted with syngeneic bone marrow cells of Mmp14 −/− or Mmp14 +/+ mice. In both groups, histological analyses of the aortic root revealed similar plaque size and macrophage and smooth muscle cell content after 8 or 16 weeks of atherogenic diet. By 16 weeks, however, the plaques of low-density lipoprotein receptor–deficient mice engrafted with Mmp14 −/− bone marrow (n=12) contained significantly more interstitial collagen than those receiving Mmp14 +/+ bone marrow (n=14; P Mmp14 −/− mice had significantly less interstitial collagenase activity than those from Mmp14 +/+ mice both basally ( P P Conclusion— MMP-14 from bone marrow–derived cells can influence the collagen content of mouse atheroma, a critical component of plaque stability. |
| Databáze: | OpenAIRE |
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