Uptake of maternal immunoglobulins in the enterocytes of suckling piglets: improved detection with a streptavidin-biotin bridge gold technique
| Autor: | J P Heath, L G Kömüves |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Streptavidin
Histology Brush border Swine Enterocyte Biotin Immunoglobulins Biology Intestinal absorption Immunolabeling chemistry.chemical_compound Bacterial Proteins medicine Animals Apical cytoplasm Colostrum Molecular biology Microscopy Electron Jejunum medicine.anatomical_structure Animals Newborn Intestinal Absorption chemistry Biochemistry Biotinylation Gold Anatomy Immunity Maternally-Acquired |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 40:1637-1646 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/40.11.1431052 |
| Popis: | In ungulates, intestinal absorption of maternal immunoglobulins from colostrum plays a vital role in the acquisition of passive immunity during early neonatal life. In the present study we used post-embedding colloidal gold labeling to examine the intracellular localization of IgG in the jejunal enterocytes of miniature piglets suckled for 2 hr. Quantitation of the immunolabeling revealed that the most sensitive technique for IgG detection was the streptavidin bridge-gold technique. In this method, the LR White-embedded sections were labeled sequentially with biotinylated anti-porcine IgG, streptavidin, and biotinylated BSA conjugated to 10-nm colloidal gold. With this approach, we found the following sequence of maternal IgG accumulation: passage of IgG from colostrum through the brush border; binding to the apical plasma membrane; uptake in noncoated pits and invaginations; transport in endocytotic vesicles; and accumulation in granules in the apical cytoplasm. |
| Databáze: | OpenAIRE |
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