In vivo light sheet fluorescence microscopy of calcium oscillations in arabidopsis thaliana
| Autor: | Alex Costa, Neli Romano Armada, Gianluca Valentini, Alessia Candeo, Fabrizio Gandolfo Doccula, Andrea Bassi |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Materials science Microscope Calcium sensor Light NES-YC3.6 Image Processing Calcium oscillations Arabidopsis chemistry.chemical_element Image processing Calcium 01 natural sciences Plant Roots Fluorescence law.invention 03 medical and health sciences Calcium imaging Computer-Assisted law Calcium Signaling Cameleon In vivo plant imaging Light sheet fluorescent microscopy Image Processing Computer-Assisted Microscopy Fluorescence Optical Imaging Microscopy biology Cameleon (protein) biology.organism_classification Photobleaching 030104 developmental biology chemistry Light sheet fluorescence microscopy biology.protein Biophysics 010606 plant biology & botany |
| Zdroj: | Calcium Signalling ISBN: 9781493990177 |
| Popis: | Calcium imaging in plants requires a high-resolution microscope, able to perform volumetric acquisition in a few seconds, inducing as low photobleaching and phototoxicity as possible to the sample. Light sheet fluorescence microscopy offers these capabilities, with the further chance to mount the sample in vertical position, mimicking the plant's growth and physiological conditions.A protocol for plant preparation and mounting in a light sheet microscope is presented. First, the growth of Arabidopsis thaliana in a sample holder compatible with light sheet microscopy is described. Then, the requirements for sample alignment and image acquisition are detailed. Finally, the image processing steps to analyze calcium oscillations are discussed, with particular emphasis on ratiometric calcium imaging in Arabidopsis root hairs. |
| Databáze: | OpenAIRE |
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