Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale
| Autor: | Daniel S. Terry, Scott C. Blanchard, Hong Zhao, Michael R. Wasserman, Zhou Zhou, Manuel F. Juette, Roger B. Altman |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Time Factors Image processing Biochemistry Article 03 medical and health sciences 0302 clinical medicine Molecular recognition RNA Transfer Microscopy Fluorescence Resonance Energy Transfer Image Processing Computer-Assisted Humans Molecular Biology Physics Millisecond Bacteria Detector Cell Biology Single Molecule Imaging Molecular Imaging 030104 developmental biology Förster resonance energy transfer Microscopy Fluorescence Biophysics Molecular imaging Biological system Ribosomes 030217 neurology & neurosurgery Algorithms Biotechnology |
| Zdroj: | Nature methods |
| ISSN: | 1548-7105 1548-7091 |
| Popis: | Molecular recognition is often driven by transient processes beyond the reach of detection. Single-molecule fluorescence microscopy methods are uniquely suited for detecting such non-accumulating intermediates, yet achieving the time resolution and statistics to realize this potential has proven challenging. Here, we present a single-molecule fluorescence resonance energy transfer (smFRET) imaging and analysis platform leveraging advances in scientific complementary metal-oxide semiconductor (sCMOS) detectors that enable the imaging of more than 10,000 individual molecules simultaneously at millisecond rates. The utility of this advance is demonstrated through quantitative measurements of previously obscured processes relevant to the fidelity mechanism in protein synthesis. |
| Databáze: | OpenAIRE |
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