Estimation of photosynthesis in cyanobacteria by pulse-amplitude modulation chlorophyll fluorescence: problems and solutions
| Autor: | Takako Ogawa, Kintake Sonoike, Masahiro Misumi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cyanobacteria Chlorophyll Light Plastoquinone Plant Science Biology Photosynthesis 01 natural sciences Biochemistry Models Biological Fluorescence 03 medical and health sciences chemistry.chemical_compound Botany Chlorophyll fluorescence Blue light Plant physiology DCMU Cell Biology General Medicine biology.organism_classification 030104 developmental biology chemistry Pulse-amplitude modulation Biological system 010606 plant biology & botany |
| Zdroj: | Photosynthesis research. 133(1-3) |
| ISSN: | 1573-5079 |
| Popis: | Cyanobacteria are photosynthetic prokaryotes and widely used for photosynthetic research as model organisms. Partly due to their prokaryotic nature, however, estimation of photosynthesis by chlorophyll fluorescence measurements is sometimes problematic in cyanobacteria. For example, plastoquinone pool is reduced in the dark-acclimated samples in many cyanobacterial species so that conventional protocol developed for land plants cannot be directly applied for cyanobacteria. Even for the estimation of the simplest chlorophyll fluorescence parameter, F v/F m, some additional protocol such as addition of DCMU or illumination of weak blue light is necessary. In this review, those problems in the measurements of chlorophyll fluorescence in cyanobacteria are introduced, and solutions to those problems are given. |
| Databáze: | OpenAIRE |
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