Phenotype, function, and differentiation potential of human monocyte subsets

Autor: Diana Metes, Geetha Chalasani, John T. Walters, Juan M. Taboas, Beth Elinoff, Bala Ramaswami, Fadi G. Lakkis, Kevin Hadi, Lisa B. Boyette, Camila Macedo
Rok vydání: 2017
Předmět:
0301 basic medicine
Physiology
Cellular differentiation
Interleukin-1beta
lcsh:Medicine
Monocytes
White Blood Cells
0302 clinical medicine
Animal Cells
Interferon
Immune Physiology
Medicine and Health Sciences
Macrophage
lcsh:Science
Cells
Cultured
Staining
Innate Immune System
Multidisciplinary
T Cells
Toll-Like Receptors
Cell Staining
Cell Differentiation
Phenotype
medicine.anatomical_structure
Cytokines
Tumor necrosis factor alpha
Cellular Types
Research Article
medicine.drug
Imaging Techniques
Immune Cells
T cell
CD14
Immunology
Biology
CD16
Research and Analysis Methods
Immunophenotyping
03 medical and health sciences
Phagocytosis
Fluorescence Imaging
medicine
Humans
Secretion
Blood Cells
Interleukin-6
Tumor Necrosis Factor-alpha
Macrophage Colony-Stimulating Factor
Macrophages
lcsh:R
Granulocyte-Macrophage Colony-Stimulating Factor
Biology and Life Sciences
Dendritic Cells
Cell Biology
Molecular Development
030104 developmental biology
Microscopy
Fluorescence
Specimen Preparation and Treatment
Immune System
lcsh:Q
Physiological Processes
Developmental Biology
030215 immunology
Zdroj: PLoS ONE, Vol 12, Iss 4, p e0176460 (2017)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0176460
Popis: Human monocytes have been grouped into classical (CD14++CD16−), non-classical (CD14dimCD16++), and intermediate (CD14++CD16+) subsets. Documentation of normal function and variation in this complement of subtypes, particularly their differentiation potential to dendritic cells (DC) or macrophages, remains incomplete. We therefore phenotyped monocytes from peripheral blood of healthy subjects and performed functional studies on high-speed sorted subsets. Subset frequencies were found to be tightly controlled over time and across individuals. Subsets were distinct in their secretion of TNFα, IL-6, and IL-1β in response to TLR agonists, with classical monocytes being the most producers and non-classical monocytes the least. Monocytes, particularly those of the non-classical subtype, secreted interferon-α (IFN-α) in response to intracellular TLR3 stimulation. After incubation with IL-4 and GM-CSF, classical monocytes acquired monocyte-derived DC (mo-DC) markers and morphology and stimulated allogeneic T cell proliferation in MLR; intermediate and non-classical monocytes did not. After incubation with IL-3 and Flt3 ligand, no subset differentiated to plasmacytoid DC. After incubation with GM-CSF (M1 induction) or macrophage colony-stimulating factor (M-CSF) (M2 induction), all subsets acquired macrophage morphology, secreted macrophage-associated cytokines, and displayed enhanced phagocytosis. From these studies we conclude that classical monocytes are the principal source of mo-DCs, but all subsets can differentiate to macrophages. We also found that monocytes, in particular the non-classical subset, represent an alternate source of type I IFN secretion in response to virus-associated TLR agonists.
Databáze: OpenAIRE
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