Cultural conditions on the production of extracellular enzymes by Trichoderma isolates from tobacco rhizosphere
| Autor: | K. Siva Raju, H. Ravisankar, K.L.N. Mallikharjuna Rao |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0106 biological sciences
0301 basic medicine Sclerotium Sucrose Nitrogen lcsh:QR1-502 Chitin 01 natural sciences Microbiology lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound Tobacco rhizosphere Cell Wall Tobacco Environmental Microbiology Pythium aphanidermatum Mycelium Soil Microbiology Trichoderma Rhizosphere biology Basidiomycota Chitinases Temperature Chitinase β-1 3-Glucanase Glucan 1 3-beta-Glucosidase Hydrogen-Ion Concentration biology.organism_classification Carbon Cultural conditions Culture Media 030104 developmental biology chemistry biology.protein 010606 plant biology & botany |
| Zdroj: | Brazilian Journal of Microbiology, Vol 47, Iss 1, Pp 25-32 (2016) Brazilian Journal of Microbiology |
| ISSN: | 1517-8382 |
| DOI: | 10.1016/j.bjm.2015.11.007 |
| Popis: | Twelve isolates of Trichoderma spp. isolated from tobacco rhizosphere were evaluated for their ability to produce chitinase and β-1,3-glucanase extracellular hydrolytic enzymes. Isolates ThJt1 and TvHt2, out of 12 isolates, produced maximum activities of chitinase and β-1,3-glucanase, respectively. In vitro production of chitinase and β-1,3-glucanase by isolates ThJt1 and TvHt2 was tested under different cultural conditions. The enzyme activities were significantly influenced by acidic pH and the optimum temperature was 30 °C. The chitin and cell walls of Sclerotium rolfsii, as carbon sources, supported the maximum and significantly higher chitinase activity by both isolates. The chitinase activity of isolate ThJt1 was suppressed significantly by fructose (80.28%), followed by glucose (77.42%), whereas the β-1,3-glucanase activity of ThJt1 and both enzymes of isolate TvHt2 were significantly suppressed by fructose, followed by sucrose. Ammonium nitrate as nitrogen source supported the maximum activity of chitinase in both isolates, whereas urea was a poor nitrogen source. Production of both enzymes by the isolates was significantly influenced by the cultural conditions. Thus, the isolates ThJt1 and TvHt2 showed higher levels of chitinase and β-1,3-glucanase activities and were capable of hydrolyzing the mycelium of S. rolfsii infecting tobacco. These organisms can be used therefore for assessment of their synergism in biomass production and biocontrol efficacy and for their field biocontrol ability against S. rolfsii and Pythium aphanidermatum infecting tobacco. |
| Databáze: | OpenAIRE |
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